STMN1 sirna

STMN1 siRNA (Mouse)

Cat. Num. AAA8229681

• Gene Names: Stmn1; 19k; Lag; P18; P19; Pig; Smn; Op18; Pp17; Pp18; Pp19; Pr22; Lap18
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: STMN1; STMN1 siRNA (Mouse); LAG; LAP18; PR22; Stathmin; Leukemia-associated gene protein; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; Op18; Phosphoprotein p19; pp19; Prosolin; Protein Pr22; pp17; STMN1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: STMN1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 149

• Applicable Applications for STMN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse STMN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for STMN1 sirna

siRNA to inhibit STMN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9789995
• NCBI GeneID: 16765
• NCBI Accession #: NP_062615.1
• NCBI GenBank Nucleotide #: NM_019641.4
• UniProt Accession #: P54227
• Molecular Weight: 17,274 Da
• NCBI Official Full Name: stathmin
• NCBI Official Synonym Full Names: stathmin 1
• NCBI Official Symbol: Stmn1
• NCBI Official Synonym Symbols: 19k; Lag; P18; P19; Pig; Smn; Op18; Pp17; Pp18; Pp19; Pr22; Lap18
• NCBI Protein Information: stathmin
• UniProt Protein Name: Stathmin
• Protein Family: Stathmin
• UniProt Gene Name: Stmn1
• UniProt Synonym Gene Names: Lag; Lap18; Pr22; Op18; pp19
• UniProt Entry Name: STMN1_MOUSE

Uniprot Description

STMN1: a microtubule-associated protein involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Phosphorylation reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, nonleukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

Protein type: Cytoskeletal

Cellular Component: microtubule; neuron projection; cytoskeleton; membrane; cytoplasm; intracellular; cytosol

Molecular Function: tubulin binding; protein binding

Biological Process: nervous system development; mitotic spindle organization and biogenesis; axonogenesis; multicellular organismal development; negative regulation of microtubule polymerization; microtubule depolymerization; positive regulation of cell motility; regulation of microtubule polymerization or depolymerization; cell differentiation