SON sirna

SON siRNA (Mouse)

Cat. Num. AAA8222540

• Gene Names: Son; nrebp; C81487; AA409051; AU067731; mKIAA1019; 2900011L12Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SON; SON siRNA (Mouse); NREBP; Protein SON; Negative regulatory element-binding protein; NRE-binding protein; SON sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SON siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2444

• Applicable Applications for SON sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SON gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SON sirna

siRNA to inhibit SON expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124358955
• NCBI GeneID: 20658
• NCBI Accession #: NP_849211.3
• NCBI GenBank Nucleotide #: NM_178880.4
• UniProt Accession #: Q9QX47; Q811G3; Q9CQ12; Q9CQK6; E3WC91; E9PXW2; E9Q3H8; E9Q3I0; E9Q6M4; E9Q7G2; E9QAR8; E9QMT0
• Molecular Weight: 247,971 Da
• NCBI Official Full Name: protein SON isoform 1
• NCBI Official Synonym Full Names: Son DNA binding protein
• NCBI Official Symbol: Son
• NCBI Official Synonym Symbols: nrebp; C81487; AA409051; AU067731; mKIAA1019; 2900011L12Rik
• NCBI Protein Information: protein SON
• UniProt Protein Name: Protein SON
• Protein Family: Son of sevenless
• UniProt Gene Name: Son
• UniProt Synonym Gene Names: Nrebp; NRE-binding protein
• UniProt Entry Name: SON_MOUSE

Uniprot Description

SON: RNA-binding protein that acts as a mRNA splicing cofactor by promoting efficient splicing of transcripts that posses weak splice sites. Specifically promotes splicing of many cell-cycle and DNA-repair transcripts that posses weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG. Probably acts by facilitating the interaction between Serine/arginine-rich proteins such as SRSF2 and the RNA polymerase II. Also binds to DNA; binds to the consensus DNA sequence: 5'- GA[GT]AN[CG][AG]CC-3'. May indirectly repress hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; RNA-binding

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: protein binding; RS domain binding; DNA binding; nucleic acid binding; RNA binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of cell cycle; regulation of RNA splicing; RNA splicing; cytokinesis; microtubule cytoskeleton organization and biogenesis; mRNA processing; cell cycle; negative regulation of apoptosis