SLC22A18 sirna

SLC22A18 siRNA (Mouse)

Cat. Num. AAA829840

• Gene Names: Slc22a18; HET; ITM; BWR1A; Impt1; TSSC5; Orctl2; BWSCR1A; AW260131; Slc22a1l; p45-BWR1A
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC22A18; SLC22A18 siRNA (Mouse); IMPT1; ITM; ORCTL2; TSSC5; Solute carrier family 22 member 18; Imprinted multi-membrane-spanning polyspecific transporter-related protein 1; Multi-membrane-spanning polyspecific transporter; Organic cation transporter-like protein 2; ORCTL-2; SLC22A18 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SLC22A18 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 410

• Applicable Applications for SLC22A18 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC22A18 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC22A18 sirna

siRNA to inhibit SLC22A18 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 112363086
• NCBI GeneID: 18400
• NCBI Accession #: NP_001036225.1
• NCBI GenBank Nucleotide #: NM_001042760.1
• UniProt Accession #: Q78KK3; O35428; O54959; O88283; Q99NF4
• Molecular Weight: 43,013 Da
• NCBI Official Full Name: solute carrier family 22 member 18
• NCBI Official Synonym Full Names: solute carrier family 22 (organic cation transporter), member 18
• NCBI Official Symbol: Slc22a18
• NCBI Official Synonym Symbols: HET; ITM; BWR1A; Impt1; TSSC5; Orctl2; BWSCR1A; AW260131; Slc22a1l; p45-BWR1A
• NCBI Protein Information: solute carrier family 22 member 18
• UniProt Protein Name: Solute carrier family 22 member 18
• Protein Family: Solute carrier family
• UniProt Gene Name: Slc22a18
• UniProt Synonym Gene Names: Impt1; Itm; Orctl2; Tssc5; Multi-membrane-spanning polyspecific transporter; ORCTL-2
• UniProt Entry Name: S22AI_MOUSE

Uniprot Description

SLC22A18: May act as a transporter of organic cations based on a proton efflux antiport mechanism. May play a role in the transport of chloroquine and quinidine-related compounds in kidney. Defects in SLC22A18 are associated with lung cancer (LNCR). LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis. Defects in SLC22A18 are a cause of rhabdomyosarcoma type 1 (RMS1). It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; cytoplasm; apical plasma membrane; plasma membrane; integral to membrane; nuclear envelope

Molecular Function: transporter activity; ubiquitin protein ligase binding; symporter activity; organic anion transmembrane transporter activity; drug transporter activity

Biological Process: drug transport; transport; multidrug transport; ion transport; transmembrane transport; anion transport; organic anion transport

Disease: Breast Cancer