Rabbit anti-Human WEE1 Polyclonal Antibody | anti-WEE1 antibody

WEE1, CT (Wee1-like Protein Kinase, Wee1A Kinase, WEE1hu) (Biotin)

Cat. Num. AAA6505973

• Gene Names: WEE1; WEE1A; WEE1hu
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: WEE1; Polyclonal Antibody; CT (Wee1-like Protein Kinase; Wee1A Kinase; WEE1hu) (Biotin); anti-WEE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WEE1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-WEE1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human WEE1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-WEE1 Pab in HL-60 cell lysate. WEE1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-WEE1 antibody

Antibodies; Protein Kinases

References

1. Kawasaki, H., et al., Oncogene 22(44):6839-6844 (2003). 2. Hashimoto, O., et al., Mol. Carcinog. 36(4):171-182 (2003). 3. Yuan, H., et al., J. Virol. 77(3):2063-2070 (2003). 4. Masaki, T., et al., Hepatology 37(3):534-543 (2003). 5. de Noronha, C.M., et al., Science 294(5544):1105-1108 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 221307502
• NCBI GeneID: 7465
• NCBI Accession #: NP_001137448.1
• NCBI GenBank Nucleotide #: NM_001143976.1
• UniProt Accession #: P30291; B3KVE1; D3DQV0
• Molecular Weight: 71,597 Da
• NCBI Official Full Name: wee1-like protein kinase isoform 2
• NCBI Official Synonym Full Names: WEE1 homolog (S. pombe)
• NCBI Official Symbol: WEE1
• NCBI Official Synonym Symbols: WEE1A; WEE1hu
• NCBI Protein Information: wee1-like protein kinase; wee1A kinase; WEE1+ homolog
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: WEE1
• UniProt Synonym Gene Names: WEE1hu
• UniProt Entry Name: WEE1_HUMAN

NCBI Description

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.10.2; Kinase, protein; Protein kinase, Other; Other group; WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: mitosis; peptidyl-tyrosine phosphorylation; establishment of cell polarity; regulation of cell cycle; cell division; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; neurite morphogenesis; G1/S transition of mitotic cell cycle