SIGLEC12 sirna

SIGLEC12 siRNA (Mouse)

Cat. Num. AAA8204277

• Gene Names: Siglece; Cd170; Siglec5; Siglec9; Siglec12; Siglecl1; mSiglec-E
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SIGLEC12; SIGLEC12 siRNA (Mouse); SIGLEC5; SIGLECE; SIGLECL1; Sialic acid-binding Ig-like lectin 12; Siglec-12; Myeloid inhibitory siglec; MIS; Sialic acid-binding Ig-like lectin 5; Siglec-5; Sialic acid-binding Ig-like lectin E; Siglec-E; mSiglec-E; Sialic acid-binding Ig-like lectin-like 1; Siglec-L1; SIGLEC12 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SIGLEC12 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 467

• Applicable Applications for SIGLEC12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SIGLEC12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SIGLEC12 sirna

siRNA to inhibit SIGLEC12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 134032002
• NCBI GeneID: 83382
• NCBI Accession #: NP_112458.2
• NCBI GenBank Nucleotide #: NM_031181.2
• UniProt Accession #: Q91Y57; E9QMD1
• Molecular Weight: 51,901 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 12
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin E
• NCBI Official Symbol: Siglece
• NCBI Official Synonym Symbols: Cd170; Siglec5; Siglec9; Siglec12; Siglecl1; mSiglec-E
• NCBI Protein Information: sialic acid-binding Ig-like lectin 12
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 12
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: Siglec12
• UniProt Synonym Gene Names: Siglec5; Siglece; Siglecl1; Siglec-12; MIS; Siglec-5; Siglec-E; mSiglec-E; Siglec-L1
• UniProt Entry Name: SIG12_MOUSE

Uniprot Description

SIGLEC7: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: monosaccharide binding; receptor signaling protein activity; receptor activity; carbohydrate binding; sialic acid binding

Biological Process: negative regulation of phagocytosis, engulfment; negative regulation of inflammatory response; cell adhesion