SCN1B sirna

SCN1B siRNA (Mouse)

Cat. Num. AAA827864

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCN1B; SCN1B siRNA (Mouse); Sodium channel subunit beta-1; SCN1B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SCN1B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 218

• Applicable Applications for SCN1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SCN1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCN1B sirna

siRNA to inhibit SCN1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755408
• NCBI GeneID: 20266
• NCBI Accession #: NP_035452.1
• NCBI GenBank Nucleotide #: NM_011322.2
• UniProt Accession #: P97952
• Molecular Weight: 24,650 Da
• NCBI Official Full Name: sodium channel subunit beta-1
• NCBI Official Synonym Full Names: sodium channel, voltage-gated, type I, beta
• NCBI Official Symbol: Scn1b
• NCBI Protein Information: sodium channel subunit beta-1
• UniProt Protein Name: Sodium channel subunit beta-1
• Protein Family: Sodium channel
• UniProt Gene Name: Scn1b
• UniProt Entry Name: SCN1B_MOUSE

Uniprot Description

SCN1B: Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. The subunit beta-1 can modulate multiple alpha subunit isoforms from brain, skeletal muscle, and heart. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN1B are the cause of generalized epilepsy with febrile seizures plus type 1 (GEFS+1). Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity. Defects in SCN1B are the cause of Brugada syndrome type 5 (BRGDA5). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN1B (TC 8.A.17) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, sodium; Membrane protein, integral

Cellular Component: voltage-gated sodium channel complex; membrane; T-tubule; integral to membrane

Molecular Function: sodium channel inhibitor activity; protein binding; sodium channel regulator activity; voltage-gated sodium channel activity; voltage-gated ion channel activity; sodium channel activity

Biological Process: membrane depolarization; axon guidance; transport; corticospinal neuron axon guidance; action potential propagation; sodium ion transport; ion transport; regulation of sodium ion transport; locomotion; response to pyrethroid; cardiac muscle contraction