SALL4 sirna

SALL4 siRNA (Mouse)

Cat. Num. AAA8218105

• Gene Names: Sall4; Tex20; C78083; C78563; AA407717; AL022809; AW536104; 5730441M18Rik; C330011P20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SALL4; SALL4 siRNA (Mouse); Sal-like protein 4; Zinc finger protein SALL4; SALL4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SALL4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1067

• Applicable Applications for SALL4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SALL4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SALL4 sirna

siRNA to inhibit SALL4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 117553631
• NCBI GeneID: 99377
• NCBI Accession #: NP_780512.2
• NCBI GenBank Nucleotide #: NM_175303.4
• UniProt Accession #: Q8BX22; Q6S7E8; Q6S7E9; Q7TST6; A2AV00
• Molecular Weight: 29,725 Da
• NCBI Official Full Name: sal-like protein 4 isoform a
• NCBI Official Synonym Full Names: sal-like 4 (Drosophila)
• NCBI Official Symbol: Sall4
• NCBI Official Synonym Symbols: Tex20; C78083; C78563; AA407717; AL022809; AW536104; 5730441M18Rik; C330011P20Rik
• NCBI Protein Information: sal-like protein 4
• UniProt Protein Name: Sal-like protein 4
• Protein Family: Sal-like protein
• UniProt Gene Name: Sall4
• UniProt Entry Name: SALL4_MOUSE

NCBI Description

This gene belongs to the spalt family of zinc finger transcription factors. In mouse, functions for this gene have been described in many embryonic developmental processes, including brain, heart, and limb development. In addition, this gene is an important pluripotency factor that is required for stem cell maintenance. Homozygous mutant mice display embryonic lethality, while conditional knock-out in embryonic germ cells results in failure to establish a robust stem cell population. A pseudogene of this gene is found on chromosome 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

SALL4: a transcription factor and oncofetal protein expressed embryonically and in the testis. Plays a key role in maintenance and self-renewal of embryonic stem cells. Interacts with NANOG and OCT4. Ectopically expressed in acute B-cell lymphoblastic leukemia (B-ALL) and myeloid leukemia (AML), playing a role in the survival of B-ALL cells and in myeloid leukemogenesis. Aberrantly expressed in multiple carcinomas including human gastric carcinoma and hepatocellular carcinoma. Maintains the stem cell qualities of EpCAM-positive hepatocellular carcinoma cells. Expressed in primary human endometrial cancer samples but not normal or hyperplastic endometrial cells. Its expression in carcinomas positively correlates with metastatic potential and poor patient survival. Specifically binds the c-Myc promoter region in endometrial cancer cells, apparently increasing c-Myc expression. Belongs to the sal C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cancer Testis Antigen (CTA); DNA-binding

Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; protein complex; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: protein binding; nucleic acid binding; DNA binding; metal ion binding

Biological Process: neural tube development; tissue development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; in utero embryonic development; heart development; stem cell maintenance; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inner cell mass cell proliferation; embryonic limb morphogenesis