RYBP sirna

RYBP siRNA (Mouse)

Cat. Num. AAA8227166

• Gene Names: Rybp; DEDAF; YEAF1; 2410018J24Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RYBP; RYBP siRNA (Mouse); DEDAF; RING1 and YY1-binding protein; Death effector domain-associated factor; DED-associated factor; RYBP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RYBP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 228

• Applicable Applications for RYBP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RYBP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RYBP sirna

siRNA to inhibit RYBP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38348308
• NCBI GeneID: 56353
• NCBI Accession #: NP_062717.2
• NCBI GenBank Nucleotide #: NM_019743.3
• UniProt Accession #: Q8CCI5; Q9WVK2; B2RRB6
• Molecular Weight: 24,777 Da
• NCBI Official Full Name: RING1 and YY1-binding protein
• NCBI Official Synonym Full Names: RING1 and YY1 binding protein
• NCBI Official Symbol: Rybp
• NCBI Official Synonym Symbols: DEDAF; YEAF1; 2410018J24Rik
• NCBI Protein Information: RING1 and YY1-binding protein
• UniProt Protein Name: RING1 and YY1-binding protein
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: Rybp
• UniProt Synonym Gene Names: Dedaf; DED-associated factor
• UniProt Entry Name: RYBP_MOUSE

Uniprot Description

RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.

Protein type: Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; cytoplasm; PcG protein complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; metal ion binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; negative regulation of transcription from RNA polymerase II promoter