RTF1 sirna

RTF1 siRNA (Mouse)

Cat. Num. AAA8205946

• Gene Names: Rtf1; Gtl7; cbp82; AI853581; AU043144; AW553985; 2900005O08Rik; 6530416A09Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RTF1; RTF1 siRNA (Mouse); RNA polymerase-associated protein RTF1 homolog; RTF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RTF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 715

• Applicable Applications for RTF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RTF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RTF1 sirna

siRNA to inhibit RTF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 195976788
• NCBI GeneID: 76246
• NCBI Accession #: NP_084388.2
• NCBI GenBank Nucleotide #: NM_030112.2
• UniProt Accession #: A2AQ19; Q08EC5
• Molecular Weight: 80,797 Da
• NCBI Official Full Name: RNA polymerase-associated protein RTF1 homolog
• NCBI Official Synonym Full Names: Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• NCBI Official Symbol: Rtf1
• NCBI Official Synonym Symbols: Gtl7; cbp82; AI853581; AU043144; AW553985; 2900005O08Rik; 6530416A09Rik
• NCBI Protein Information: RNA polymerase-associated protein RTF1 homolog
• UniProt Protein Name: RNA polymerase-associated protein RTF1 homolog
• Protein Family: Replication termination factor
• UniProt Gene Name: Rtf1
• UniProt Entry Name: RTF1_MOUSE

Uniprot Description

RTF1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single- stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.

Protein type: Transcription factor

Cellular Component: nucleolus; nucleus

Molecular Function: DNA binding; single-stranded DNA binding

Biological Process: Wnt receptor signaling pathway; transcription, DNA-dependent; endodermal cell fate commitment; regulation of transcription, DNA-dependent; positive regulation of RNA elongation from RNA polymerase II promoter; histone modification; stem cell maintenance; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; blastocyst growth