RPS24 sirna

RPS24 siRNA (Mouse)

Cat. Num. AAA8236792

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RPS24; RPS24 siRNA (Mouse); 40S ribosomal protein S24; RPS24 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RPS24 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 131

• Applicable Applications for RPS24 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RPS24 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RPS24 sirna

siRNA to inhibit RPS24 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46519156
• NCBI GeneID: 20088
• NCBI Accession #: NP_035427.2
• NCBI GenBank Nucleotide #: NM_011297.2
• UniProt Accession #: P62849; P16632; Q642L0; Q6PDB5
• Molecular Weight: 15,197 Da
• NCBI Official Full Name: 40S ribosomal protein S24 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein S24
• NCBI Official Symbol: Rps24
• NCBI Protein Information: 40S ribosomal protein S24
• UniProt Protein Name: 40S ribosomal protein S24
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: Rps24
• UniProt Entry Name: RS24_MOUSE

Uniprot Description

RPS24: Required for processing of pre-rRNA and maturation of 40S ribosomal subunits. Defects in RPS24 are the cause of Diamond-Blackfan anemia type 3 (DBA3). DBA3 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein S24e family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation; Ribosomal

Cellular Component: small ribosomal subunit; membrane; cytoplasm; nucleolus; ribosome; intracellular; ribonucleoprotein complex; nucleus

Molecular Function: structural constituent of ribosome; translation initiation factor binding; nucleotide binding

Biological Process: erythrocyte homeostasis; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ribosomal small subunit biogenesis and assembly; translational elongation; translation; rRNA processing