ROS1 sirna

ROS1 siRNA (Mouse)

Cat. Num. AAA829043

• Gene Names: Ros1; Ros-1; c-ros
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ROS1; ROS1 siRNA (Mouse); ROS; ROS-1; Proto-oncogene tyrosine-protein kinase ROS; Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase; ROS1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ROS1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2340

• Applicable Applications for ROS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ROS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ROS1 sirna

siRNA to inhibit ROS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 114326510
• NCBI GeneID: 19886
• NCBI Accession #: NP_035412.2
• NCBI GenBank Nucleotide #: NM_011282.2
• UniProt Accession #: Q78DX7; Q60705
• Molecular Weight: 261,967 Da
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase ROS
• NCBI Official Synonym Full Names: Ros1 proto-oncogene
• NCBI Official Symbol: Ros1
• NCBI Official Synonym Symbols: Ros-1; c-ros
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase ROS
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase ROS
• Protein Family: Protein
• UniProt Gene Name: Ros1
• UniProt Synonym Gene Names: Ros; Ros-1
• UniProt Entry Name: ROS1_MOUSE

Uniprot Description

ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in adult murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma.

Protein type: Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; Protein kinase, TK; TK group; Sev family

Cellular Component: cell surface; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane

Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: cell proliferation; peptidyl-tyrosine phosphorylation; spermatogenesis; cell differentiation; columnar/cuboidal epithelial cell development; cell growth; protein amino acid phosphorylation; phosphorylation; regulation of TOR signaling pathway