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Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 5 % SDS PAGE gel and INPP5F antibody at a dilution of 1:500)

Rabbit INPP5F Polyclonal Antibody | anti-INPP5F antibody

INPP5F antibody

Gene Names
INPP5F; SAC2; hSAC2; MSTP007; MSTPO47
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
INPP5F antibody was purified by antigen-affinity chromatography
Synonyms
INPP5F; Polyclonal Antibody; INPP5F antibody; Polyclonal INPP5F; Anti-INPP5F; FLJ13081; KIAA0966; INPPF 5; MSTPO47; INPPF-5; MGC131851; SAC2; MGC59773; hSAC2; anti-INPP5F antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
INPP5F antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.79 mg/ml (varies by lot)
Sequence Length
434
Applicable Applications for anti-INPP5F antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:1000
ICC:1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Biological Significance
The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate.
Cross-Reactivity
Human, Mouse
Immunogen
INPP5F antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 593 and 904 of INPP5F
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 5 % SDS PAGE gel and INPP5F antibody at a dilution of 1:500)

Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 5 % SDS PAGE gel and INPP5F antibody at a dilution of 1:500)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and INPP5F antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and INPP5F antibody at a dilution of 1:1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed HeLa, using INPP5F antibody at 1:200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed HeLa, using INPP5F antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded Colon ca using INPP5F antibody at a dilution of 1:500)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded Colon ca using INPP5F antibody at a dilution of 1:500)
Related Product Information for anti-INPP5F antibody
Affinity purified Rabbit polyclonal INPP5F antibody
Product Categories/Family for anti-INPP5F antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
128 kDa
NCBI Official Full Name
INPP5F protein, partial
NCBI Official Synonym Full Names
inositol polyphosphate-5-phosphatase F
NCBI Official Symbol
INPP5F
NCBI Official Synonym Symbols
SAC2; hSAC2; MSTP007; MSTPO47
NCBI Protein Information
phosphatidylinositide phosphatase SAC2
UniProt Protein Name
Phosphatidylinositide phosphatase SAC2
UniProt Gene Name
INPP5F
UniProt Synonym Gene Names
KIAA0966; SAC2; hSAC2
UniProt Entry Name
SAC2_HUMAN

NCBI Description

The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

INPP5F: Inositol 5-phosphatase which acts on phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Hydrolyzes phosphatidylinositol 4,5- bisphosphate most effectively. Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase, lipid; EC 3.1.3.-

Chromosomal Location of Human Ortholog: 10q26.11

Molecular Function: phosphoric ester hydrolase activity

Biological Process: phosphatidylinositol catabolic process; cardiac muscle hypertrophy; phosphoinositide-mediated signaling; regulation of protein kinase B signaling cascade

Research Articles on INPP5F

Similar Products

Product Notes

The INPP5F inpp5f (Catalog #AAA835282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's INPP5F can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:1000 ICC:1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the INPP5F inpp5f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INPP5F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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