RIPK2 sirna

RIPK2 siRNA (Mouse)

Cat. Num. AAA8208019

• Gene Names: Ripk2; CCK; RICK; RIP2; CARD3; CARDIAK; D4Bwg0615e; 2210420D18Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RIPK2; RIPK2 siRNA (Mouse); Receptor-interacting serine/threonine-protein kinase 2; Tyrosine-protein kinase RIPK2; RIPK2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RIPK2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 539

• Applicable Applications for RIPK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RIPK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RIPK2 sirna

siRNA to inhibit RIPK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20336736
• NCBI GeneID: 192656
• NCBI Accession #: NP_620402.1
• NCBI GenBank Nucleotide #: NM_138952.3
• UniProt Accession #: P58801
• Molecular Weight: 60,400 Da
• NCBI Official Full Name: receptor-interacting serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: receptor (TNFRSF)-interacting serine-threonine kinase 2
• NCBI Official Symbol: Ripk2
• NCBI Official Synonym Symbols: CCK; RICK; RIP2; CARD3; CARDIAK; D4Bwg0615e; 2210420D18Rik
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 2
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: Ripk2
• UniProt Entry Name: RIPK2_MOUSE

Uniprot Description

RIPK2: a tyrosine kinase-like kinase of the RIPK family. Activates pro-caspase-1 and pro-caspase-8. Potentiates casp-8-mediated apoptosis. May activate NF-kappaB.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.10.2; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family

Cellular Component: protein complex; cytoskeleton; cytoplasm; intracellular; vesicle

Molecular Function: transferase activity; protein serine/threonine kinase activity; LIM domain binding; protein homodimerization activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; CARD domain binding; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of T-helper 1 type immune response; apoptosis; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of caspase activity; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; activation of NF-kappaB transcription factor; T cell proliferation; regulation of apoptosis; response to exogenous dsRNA; positive regulation of immature T cell proliferation; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; adaptive immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of peptidyl-serine phosphorylation; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; positive regulation of protein ubiquitination; positive regulation of interleukin-2 production; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T-helper 1 cell differentiation; phosphorylation