RGS14 sirna

RGS14 siRNA (Mouse)

Cat. Num. AAA8238481

• Gene Names: Rgs14; RPIP1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RGS14; RGS14 siRNA (Mouse); Regulator of G-protein signaling 14; RAP1/RAP2-interacting protein; RPIP1; RGS14 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RGS14 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 547

• Applicable Applications for RGS14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RGS14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RGS14 sirna

siRNA to inhibit RGS14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31980626
• NCBI GeneID: 51791
• NCBI Accession #: NP_058038.2
• NCBI GenBank Nucleotide #: NM_016758.3
• UniProt Accession #: P97492; Q8K2R4; Q9DCD1
• Molecular Weight: 59,847 Da
• NCBI Official Full Name: regulator of G-protein signaling 14
• NCBI Official Synonym Full Names: regulator of G-protein signaling 14
• NCBI Official Symbol: Rgs14
• NCBI Official Synonym Symbols: RPIP1
• NCBI Protein Information: regulator of G-protein signaling 14
• UniProt Protein Name: Regulator of G-protein signaling 14
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: Rgs14
• UniProt Synonym Gene Names: RGS14; RPIP1
• UniProt Entry Name: RGS14_MOUSE

Uniprot Description

RGS14: Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, RGS

Cellular Component: spindle pole; centrosome; microtubule; dendrite; postsynaptic density; dendritic spine; nuclear body; postsynaptic membrane; cell projection; cytoskeleton; membrane; cytoplasm; plasma membrane; synapse; spindle; nucleus; cell junction

Molecular Function: protein binding; receptor signaling complex scaffold activity; microtubule binding; GDP-dissociation inhibitor activity; receptor signaling protein activity; GTPase activating protein binding; G-protein alpha-subunit binding; GTPase regulator activity; protein kinase binding; GTPase activator activity

Biological Process: regulation of transcription in response to stress; negative regulation of MAP kinase activity; mitosis; zygote asymmetric cell division; positive regulation of neurogenesis; negative regulation of synaptic plasticity; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; nucleocytoplasmic transport; learning; signal transduction; cell cycle; spindle organization and biogenesis; negative regulation of signal transduction; chromosome segregation; G-protein coupled receptor protein signaling pathway; long-term memory; cell division; visual learning; response to oxidative stress; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity