RB1 sirna

RB1 siRNA (Mouse)

Cat. Num. AAA8206723

• Gene Names: Rb1; Rb; pRb; Rb-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RB1; RB1 siRNA (Mouse); RB-1; Retinoblastoma-associated protein; pRb; Rb; pp105; RB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 921

• Applicable Applications for RB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RB1 sirna

siRNA to inhibit RB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 188528630
• NCBI GeneID: 19645
• NCBI Accession #: NP_033055.2
• NCBI GenBank Nucleotide #: NM_009029.2
• UniProt Accession #: P13405; Q4VA62
• Molecular Weight: 105,367 Da
• NCBI Official Full Name: retinoblastoma-associated protein
• NCBI Official Synonym Full Names: retinoblastoma 1
• NCBI Official Symbol: Rb1
• NCBI Official Synonym Symbols: Rb; pRb; Rb-1
• NCBI Protein Information: retinoblastoma-associated protein
• UniProt Protein Name: Retinoblastoma-associated protein
• Protein Family: RB1-inducible coiled-coil protein
• UniProt Gene Name: Rb1
• UniProt Synonym Gene Names: Rb-1; Rb
• UniProt Entry Name: RB_MOUSE

Uniprot Description

Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.

Protein type: Tumor suppressor; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Oncoprotein

Cellular Component: Rb-E2F complex; transcription factor complex; PML body; spindle; nucleus

Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; ubiquitin protein ligase binding; phosphoprotein binding; kinase binding; transcription factor binding

Biological Process: sister chromatid biorientation; regulation of cell cycle; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; neuron maturation; negative regulation of cell cycle; neuron differentiation; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; negative regulation of mitotic cell cycle; positive regulation of macrophage differentiation; cell cycle arrest; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; regulation of lipid kinase activity; enucleate erythrocyte differentiation; chromatin modification; cell cycle; myoblast differentiation; regulation of transcription from RNA polymerase II promoter; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle