RASSF1 sirna

RASSF1 siRNA (Mouse)

Cat. Num. AAA8210128

• Gene Names: Rassf1; 123F2; RDA32; NORE2A; REH3P21; Rassf1A; Rassf1B; Rassf1C; AA536941; AU044980
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RASSF1; RASSF1 siRNA (Mouse); Ras association domain-containing protein 1; Protein 123F2; RASSF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RASSF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 340

• Applicable Applications for RASSF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RASSF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RASSF1 sirna

siRNA to inhibit RASSF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 344217757
• NCBI GeneID: 56289
• NCBI Accession #: NP_001230677.1
• NCBI GenBank Nucleotide #: NM_001243748.1
• UniProt Accession #: Q99MK9; Q9WUF5
• Molecular Weight: 31,193 Da
• NCBI Official Full Name: ras association domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: Ras association (RalGDS/AF-6) domain family member 1
• NCBI Official Symbol: Rassf1
• NCBI Official Synonym Symbols: 123F2; RDA32; NORE2A; REH3P21; Rassf1A; Rassf1B; Rassf1C; AA536941; AU044980
• NCBI Protein Information: ras association domain-containing protein 1
• UniProt Protein Name: Ras association domain-containing protein 1
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: Rassf1
• UniProt Entry Name: RASF1_MOUSE

Uniprot Description

RASSF1: Potential tumor suppressor. Required for death receptor- dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS1 via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform C or isoform H interacts (via N-terminus) with DAXX. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53. Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells. 8 isoforms of the human protein are produced by alternative promoter.

Protein type: Tumor suppressor

Cellular Component: microtubule cytoskeleton; microtubule; cytoskeleton; cytoplasm; plasma membrane; nucleus

Molecular Function: identical protein binding; protein binding; metal ion binding; protein N-terminus binding

Biological Process: protein stabilization; positive regulation of protein ubiquitination; Ras protein signal transduction; signal transduction; cell cycle; cell cycle arrest; response to DNA damage stimulus