RAB8B sirna

RAB8B siRNA (Mouse)

Cat. Num. AAA8211619

• Gene Names: Rab8b; 5930437D16; D330025I23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAB8B; RAB8B siRNA (Mouse); Ras-related protein Rab-8B; RAB8B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAB8B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 207

• Applicable Applications for RAB8B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAB8B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAB8B sirna

siRNA to inhibit RAB8B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27734154
• NCBI GeneID: 235442
• NCBI Accession #: NP_775589.1
• NCBI GenBank Nucleotide #: NM_173413.3
• UniProt Accession #: P61028; Q3U1Z3
• Molecular Weight: 23,603 Da
• NCBI Official Full Name: ras-related protein Rab-8B
• NCBI Official Synonym Full Names: RAB8B, member RAS oncogene family
• NCBI Official Symbol: Rab8b
• NCBI Official Synonym Symbols: 5930437D16; D330025I23Rik
• NCBI Protein Information: ras-related protein Rab-8B
• UniProt Protein Name: Ras-related protein Rab-8B
• Protein Family: Ras-related protein
• UniProt Gene Name: Rab8b
• UniProt Entry Name: RAB8B_MOUSE

Uniprot Description

RAB8B: May be involved in vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells. Belongs to the small GTPase superfamily. Rab family.

Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric, Rab; G protein, monomeric

Cellular Component: peroxisomal membrane; trans-Golgi network transport vesicle; synaptic vesicle; mitochondrion; membrane; secretory granule membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; phagocytic vesicle; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; TPR domain binding; nucleotide binding; receptor binding

Biological Process: protein secretion; positive regulation of cell projection organization and biogenesis; positive regulation of adrenocorticotropic hormone secretion; regulation of exocytosis; intracellular protein transport; antigen processing and presentation; protein transport; synaptic vesicle exocytosis; cellular response to insulin stimulus; Golgi vesicle fusion to target membrane; transport; small GTPase mediated signal transduction; Rab protein signal transduction; protein import into peroxisome membrane; vesicle docking during exocytosis