PUS1 sirna

PUS1 siRNA (Mouse)

Cat. Num. AAA8236751

• Gene Names: Pus1; MPUS1; mPus1p; A730013B20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PUS1; PUS1 siRNA (Mouse); tRNA pseudouridine synthase A mitochondrial; tRNA pseudouridine(38-40) synthase; tRNA pseudouridylate synthase I; tRNA-uridine isomerase I; PUS1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PUS1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 347

• Applicable Applications for PUS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PUS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PUS1 sirna

siRNA to inhibit PUS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70906472
• NCBI GeneID: 56361
• NCBI Accession #: NP_001020733.1
• NCBI GenBank Nucleotide #: NM_001025562.2
• UniProt Accession #: Q9WU56; Q3UUC6; Q791J1; Q8C250; Q8VDQ3; Q9EQD1; Q9JJE5
• Molecular Weight: 38,927 Da
• NCBI Official Full Name: tRNA pseudouridine synthase A, mitochondrial isoform 3
• NCBI Official Synonym Full Names: pseudouridine synthase 1
• NCBI Official Symbol: Pus1
• NCBI Official Synonym Symbols: MPUS1; mPus1p; A730013B20Rik
• NCBI Protein Information: tRNA pseudouridine synthase A, mitochondrial
• UniProt Protein Name: tRNA pseudouridine synthase A, mitochondrial
• Protein Family: tRNA pseudouridine synthase
• UniProt Gene Name: Pus1
• UniProt Entry Name: TRUA_MOUSE

Uniprot Description

PUS1: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA. Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1); also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow. Belongs to the tRNA pseudouridine synthase TruA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.4.99.12; Transcription, coactivator/corepressor; RNA-binding; Nuclear receptor co-regulator; Isomerase; RNA processing

Cellular Component: transcription factor complex; mitochondrion; nuclear chromatin; nucleolus; nucleus

Molecular Function: protein binding; beta-amyrin synthase activity; ligand-dependent nuclear receptor transcription coactivator activity; RNA binding; pseudouridine synthase activity; lupeol synthase activity; isomerase activity

Biological Process: tRNA pseudouridine synthesis; pseudouridine synthesis; tRNA processing; RNA modification; positive regulation of transcription from RNA polymerase II promoter