PTCH1 sirna

PTCH1 siRNA (Mouse)

Cat. Num. AAA8200517

• Gene Names: Ptch1; Ptc; mes; Ptc1; Ptch; A230106A15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTCH1; PTCH1 siRNA (Mouse); PTCH; Protein patched homolog 1; PTC; PTC1; PTCH1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTCH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1434

• Applicable Applications for PTCH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTCH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTCH1 sirna

siRNA to inhibit PTCH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679519
• NCBI GeneID: 19206
• NCBI Accession #: NP_032983.1
• NCBI GenBank Nucleotide #: NM_008957.2
• UniProt Accession #: Q61115
• Molecular Weight: 159,273 Da
• NCBI Official Full Name: protein patched homolog 1
• NCBI Official Synonym Full Names: patched homolog 1
• NCBI Official Symbol: Ptch1
• NCBI Official Synonym Symbols: Ptc; mes; Ptc1; Ptch; A230106A15Rik
• NCBI Protein Information: protein patched homolog 1
• UniProt Protein Name: Protein patched homolog 1
• UniProt Gene Name: Ptch1
• UniProt Synonym Gene Names: Ptch; PTC; PTC1
• UniProt Entry Name: PTC1_MOUSE

Uniprot Description

PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres.

Protein type: Cell cycle regulation; Membrane protein, integral; Tumor suppressor; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; integral to plasma membrane; perinuclear region of cytoplasm; postsynaptic density; extracellular region; integral to membrane; plasma membrane; midbody; caveola

Molecular Function: heparin binding; protein binding; cyclin binding; zinc ion binding; hedgehog receptor activity; protein complex binding; cholesterol binding; smoothened binding; patched binding

Biological Process: heart morphogenesis; hindlimb morphogenesis; epidermis development; regulation of mitotic cell cycle; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; signal transduction; response to chlorate; negative regulation of cell proliferation; regulation of protein localization; mammary gland development; regulation of growth; negative regulation of osteoblast differentiation; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; smoothened signaling pathway; epidermal cell fate specification; pharyngeal system development; negative regulation of multicellular organism growth; in utero embryonic development; negative regulation of transcription factor activity; neural tube patterning; negative regulation of cell division; keratinocyte proliferation; spinal cord motor neuron differentiation; pattern specification process; limb morphogenesis; regulation of cell proliferation; organ morphogenesis; dorsal/ventral pattern formation; ureteric bud branching; neural plate pattern formation; negative regulation of smoothened signaling pathway; neural tube closure; positive regulation of epidermal cell differentiation; embryonic organ development; protein processing; brain development; neural tube formation; regulation of smoothened signaling pathway