PRKAG2 sirna

PRKAG2 siRNA (Mouse)

Cat. Num. AAA8217913

• Gene Names: Prkag2; AAKG; WPWS; AAKG2; H91620p; AI854673; 2410051C13Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRKAG2; PRKAG2 siRNA (Mouse); 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma2; AMPK subunit gamma-2; PRKAG2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PRKAG2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 326

• Applicable Applications for PRKAG2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PRKAG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRKAG2 sirna

siRNA to inhibit PRKAG2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 282847331
• NCBI GeneID: 108099
• NCBI Accession #: NP_001164027.1
• NCBI GenBank Nucleotide #: NM_001170556.1
• UniProt Accession #: Q91WG5; Q6V7V5; E9QK80
• Molecular Weight: 37,312 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-2 isoform 3
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• NCBI Official Symbol: Prkag2
• NCBI Official Synonym Symbols: AAKG; WPWS; AAKG2; H91620p; AI854673; 2410051C13Rik
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-2
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-2
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: Prkag2
• UniProt Synonym Gene Names: AMPK gamma2; AMPK subunit gamma-2
• UniProt Entry Name: AAKG2_MOUSE

Uniprot Description

AMPKG2: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Autophagy

Cellular Component: extracellular space; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; cAMP-dependent protein kinase inhibitor activity; nucleotide binding; cAMP-dependent protein kinase regulator activity; phosphorylase kinase regulator activity; ADP binding; protein kinase activator activity; protein kinase binding; ATP binding; AMP binding

Biological Process: glycogen metabolic process; regulation of catalytic activity; regulation of glycolysis; positive regulation of protein kinase activity; regulation of fatty acid metabolic process; negative regulation of protein kinase activity; lipid metabolic process; fatty acid metabolic process; protein amino acid phosphorylation; fatty acid biosynthetic process