PRDM12 sirna

PRDM12 siRNA (Mouse)

Cat. Num. AAA8227777

• Gene Names: Prdm12; Gm998
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDM12; PRDM12 siRNA (Mouse); GM998; PR domain zinc finger protein 12; PR domain-containing protein 12; PRDM12 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PRDM12 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 365

• Applicable Applications for PRDM12 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PRDM12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDM12 sirna

siRNA to inhibit PRDM12 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 182765475
• NCBI GeneID: 381359
• NCBI Accession #: NP_001116834.1
• NCBI GenBank Nucleotide #: NM_001123362.1
• UniProt Accession #: A2AJ77
• Molecular Weight: 40,303 Da
• NCBI Official Full Name: PR domain zinc finger protein 12
• NCBI Official Synonym Full Names: PR domain containing 12
• NCBI Official Symbol: Prdm12
• NCBI Official Synonym Symbols: Gm998
• NCBI Protein Information: PR domain zinc finger protein 12
• UniProt Protein Name: PR domain zinc finger protein 12
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: Prdm12
• UniProt Synonym Gene Names: Gm998
• UniProt Entry Name: PRD12_MOUSE

Uniprot Description

PRDM12: May be involved in transcriptional regulation.

Protein type: Methyltransferase, protein lysine, predicted; C2H2-type zinc finger protein

Cellular Component: nucleus

Molecular Function: methyltransferase activity; monomethylamine methyltransferase activity; trimethylamine methyltransferase activity; tRNA (cytosine)-methyltransferase activity; RNA methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; tRNA (guanine) methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; arginine N-methyltransferase activity; selenocysteine methyltransferase activity; protein-arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; transferase activity; hydroxyneurosporene-O-methyltransferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; metal ion binding; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; tRNA (adenine)-methyltransferase activity; nucleic acid binding; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; mRNA methyltransferase activity; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; DNA binding; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity

Biological Process: methylation; transcription, DNA-dependent; neurogenesis; regulation of transcription, DNA-dependent