PPP1R13L sirna

PPP1R13L siRNA (Mouse)

Cat. Num. AAA8228880

• Gene Names: Ppp1r13l; wa3; IASPP; Nkip1; AV260376
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPP1R13L; PPP1R13L siRNA (Mouse); NKIP1; RelA-associated inhibitor; Inhibitor of ASPP protein; Protein iASPP; NFkB-interacting protein 1; PPP1R13B-like protein; PPP1R13L sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PPP1R13L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 824

• Applicable Applications for PPP1R13L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PPP1R13L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP1R13L sirna

siRNA to inhibit PPP1R13L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58082069
• NCBI GeneID: 333654
• NCBI Accession #: NP_001010836.1
• NCBI GenBank Nucleotide #: NM_001010836.3
• UniProt Accession #: Q5I1X5
• Molecular Weight: 88,976 Da
• NCBI Official Full Name: relA-associated inhibitor
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 13 like
• NCBI Official Symbol: Ppp1r13l
• NCBI Official Synonym Symbols: wa3; IASPP; Nkip1; AV260376
• NCBI Protein Information: relA-associated inhibitor
• UniProt Protein Name: RelA-associated inhibitor
• Protein Family: RelA-associated inhibitor
• UniProt Gene Name: Ppp1r13l
• UniProt Synonym Gene Names: Protein iASPP
• UniProt Entry Name: IASPP_MOUSE

Uniprot Description

PPP1R13L: an oncoprotein that interacts with and inhibits NF-kappaB, Sp1 and p53. Plays a central role in regulation of apoptosis and transcription. May inhibit p53 by preventing its association with ASPP1 or ASPP2, thereby suppressing the subsequent activation of apoptosis. Required for neuronal survival after axonal injury. Two alternatively spliced isoforms have been described. Isoform 1 is preferrentially found in the cytoplasm; isoform 2 is nuclear.

Protein type: Transcription, coactivator/corepressor; Phosphatase, regulatory subunit; Apoptosis

Cellular Component: intercellular bridge; cytoplasm; cell junction; nucleus

Molecular Function: identical protein binding; protein binding; transcription factor binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; multicellular organismal homeostasis; positive regulation of cell differentiation; embryonic camera-type eye development; post-embryonic development; cardiac muscle contraction; hair cycle