POPDC2 sirna

POPDC2 siRNA (Mouse)

Cat. Num. AAA8204186

• Gene Names: Popdc2; Pop2; AV006127
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POPDC2; POPDC2 siRNA (Mouse); POP2; Popeye domain-containing protein 2; Popeye protein 2; POPDC2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: POPDC2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 367

• Applicable Applications for POPDC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse POPDC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POPDC2 sirna

siRNA to inhibit POPDC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11612497
• NCBI GeneID: 64082
• NCBI Accession #: NP_071713.1
• NCBI GenBank Nucleotide #: NM_022318.3
• UniProt Accession #: Q9ES82
• Molecular Weight: 41,252 Da
• NCBI Official Full Name: popeye domain-containing protein 2 isoform 2
• NCBI Official Synonym Full Names: popeye domain containing 2
• NCBI Official Symbol: Popdc2
• NCBI Official Synonym Symbols: Pop2; AV006127
• NCBI Protein Information: popeye domain-containing protein 2
• UniProt Protein Name: Popeye domain-containing protein 2
• Protein Family: Popeye domain-containing
• UniProt Gene Name: Popdc2
• UniProt Synonym Gene Names: Pop2; Popeye protein 2
• UniProt Entry Name: POPD2_MOUSE

NCBI Description

This gene encodes a member of the Popeye domain containing family of membrane proteins. Proteins of this family contain three helical transmembrane domains and a conserved intracellular Popeye domain. In the adult mouse, this gene is expressed at high levels in cardiac myocytes, and mice deficient for this gene develop stress-induced cardiac pacemaker dysfunction. The protein binds to a two-pore domain potassium channel and recruits it to the plasma membrane. Cyclic adenosine monophosphate negatively regulates this interaction through the Popeye domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

POPDC2: May play an important role in heart development. Belongs to the popeye family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: membrane; integral to membrane

Molecular Function: cAMP binding

Biological Process: regulation of membrane potential; regulation of heart rate