POLH sirna

POLH siRNA (Mouse)

Cat. Num. AAA8238085

• Gene Names: Polh; XPV; RAD30A
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLH; POLH siRNA (Mouse); RAD30A; XPV; DNA polymerase eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein homolog; POLH sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: POLH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 694

• Applicable Applications for POLH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse POLH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLH sirna

siRNA to inhibit POLH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17105328
• NCBI GeneID: 80905
• NCBI Accession #: NP_109640.1
• NCBI GenBank Nucleotide #: NM_030715.3
• UniProt Accession #: Q9JJN0; Q9JJJ2
• Molecular Weight: 76,167 Da
• NCBI Official Full Name: DNA polymerase eta
• NCBI Official Synonym Full Names: polymerase (DNA directed), eta (RAD 30 related)
• NCBI Official Symbol: Polh
• NCBI Official Synonym Symbols: XPV; RAD30A
• NCBI Protein Information: DNA polymerase eta
• UniProt Protein Name: DNA polymerase eta
• Protein Family: DNA polymerase
• UniProt Gene Name: Polh
• UniProt Synonym Gene Names: Rad30a; Xpv
• UniProt Entry Name: POLH_MOUSE

Uniprot Description

POLH: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI. Belongs to the DNA polymerase type-Y family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.7.7.7; DNA repair, damage

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: transferase activity; DNA binding; metal ion binding; DNA-directed DNA polymerase activity; damaged DNA binding; nucleotidyltransferase activity

Biological Process: DNA synthesis during DNA repair; postreplication repair; pyrimidine dimer repair; DNA replication; DNA repair; response to DNA damage stimulus; response to UV-C