PNN sirna

PNN siRNA (Mouse)

Cat. Num. AAA8219236

• Gene Names: Pnn; AU045199; D12Ertd512e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PNN; PNN siRNA (Mouse); Pinin; PNN sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PNN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 726

• Applicable Applications for PNN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PNN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PNN sirna

siRNA to inhibit PNN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 112420990
• NCBI GeneID: 18949
• NCBI Accession #: NP_032917.2
• NCBI GenBank Nucleotide #: NM_008891.2
• UniProt Accession #: O35691; Q8CD89; Q8CGU3
• Molecular Weight: 82,436 Da
• NCBI Official Full Name: pinin
• NCBI Official Synonym Full Names: pinin
• NCBI Official Symbol: Pnn
• NCBI Official Synonym Symbols: AU045199; D12Ertd512e
• NCBI Protein Information: pinin
• UniProt Protein Name: Pinin
• Protein Family: Pinin
• UniProt Gene Name: Pnn
• UniProt Entry Name: PININ_MOUSE

Uniprot Description

PNN: Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma. Belongs to the pinin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Spliceosome; Tumor suppressor; Transcription, coactivator/corepressor; RNA-binding

Cellular Component: nucleoplasm; spliceosome; desmosome; membrane; cytoplasm; nuclear speck; cell junction; nucleus

Molecular Function: DNA binding

Biological Process: cell-cell adhesion; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; mRNA processing