PLSCR1 sirna

PLSCR1 siRNA (Mouse)

Cat. Num. AAA8221757

• Gene Names: Plscr1; Nor1; Tra1; Tra1a; Tra1b; Tras1; Tras2; MmTRA1a; MmTRA1b
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLSCR1; PLSCR1 siRNA (Mouse); TRA1B; TRAS1; Phospholipid scramblase 1; PL scramblase 1; Ca(2+)-dependent phospholipid scramblase 1; Transplantability-associated protein 1; NOR1; TRA1; PLSCR1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PLSCR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 328

• Applicable Applications for PLSCR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PLSCR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLSCR1 sirna

siRNA to inhibit PLSCR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194328695
• NCBI GeneID: 22038
• NCBI Accession #: NP_035766.2
• NCBI GenBank Nucleotide #: NM_011636.2
• UniProt Accession #: Q9JJ00; O54730; O54731; Q9D1F8
• Molecular Weight: 35,914 Da
• NCBI Official Full Name: phospholipid scramblase 1
• NCBI Official Synonym Full Names: phospholipid scramblase 1
• NCBI Official Symbol: Plscr1
• NCBI Official Synonym Symbols: Nor1; Tra1; Tra1a; Tra1b; Tras1; Tras2; MmTRA1a; MmTRA1b
• NCBI Protein Information: phospholipid scramblase 1
• UniProt Protein Name: Phospholipid scramblase 1
• Protein Family: Phospholipid scramblase
• UniProt Gene Name: Plscr1
• UniProt Synonym Gene Names: Tra1b; Tras1; PL scramblase 1; NOR1; TRA1
• UniProt Entry Name: PLS1_MOUSE

Uniprot Description

PLSCR1: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family.

Protein type: Calcium-binding; Membrane protein, integral

Cellular Component: extracellular matrix; Golgi apparatus; membrane; integral to plasma membrane; integral to membrane; nucleolus; plasma membrane; cytosol; nucleus; lipid raft

Molecular Function: phospholipid scramblase activity; enzyme binding; DNA binding; calcium ion binding; CD4 receptor binding; SH3 domain binding; epidermal growth factor receptor binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of apoptosis; apoptosis; phospholipid scrambling; phosphatidylserine biosynthetic process; negative regulation of viral genome replication; positive regulation of innate immune response; phospholipid transport; immune response; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation; negative regulation of protein binding; defense response to virus; regulation of mast cell activation