PITX3 sirna

PITX3 siRNA (Mouse)

Cat. Num. AAA8202798

• Gene Names: Pitx3; ak; Ptx3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PITX3; PITX3 siRNA (Mouse); Pituitary homeobox 3; Homeobox protein PITX3; Paired-like homeodomain transcription factor 3; PITX3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PITX3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 302

• Applicable Applications for PITX3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PITX3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PITX3 sirna

siRNA to inhibit PITX3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679341
• NCBI GeneID: 18742
• NCBI Accession #: NP_032878.1
• NCBI GenBank Nucleotide #: NM_008852.4
• UniProt Accession #: O35160; Q0VB03
• Molecular Weight: 31,715 Da
• NCBI Official Full Name: pituitary homeobox 3
• NCBI Official Synonym Full Names: paired-like homeodomain transcription factor 3
• NCBI Official Symbol: Pitx3
• NCBI Official Synonym Symbols: ak; Ptx3
• NCBI Protein Information: pituitary homeobox 3
• UniProt Protein Name: Pituitary homeobox 3
• Protein Family: Pituitary homeobox
• UniProt Gene Name: Pitx3
• UniProt Entry Name: PITX3_MOUSE

Uniprot Description

PITX3: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1- mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle. Defects in PITX3 are a cause of cataract autosomal dominant (ADC). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood. Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD); also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis. Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4). A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: lens development in camera-type eye; transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of gene expression; positive regulation of transcription, DNA-dependent; multicellular organismal development; midbrain development; neuron development; lens morphogenesis in camera-type eye; locomotory behavior; positive regulation of transcription from RNA polymerase II promoter