PIF1 sirna

PIF1 siRNA (Mouse)

Cat. Num. AAA8212667

• Gene Names: Pif1; AI449441; 4631410M14
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIF1; PIF1 siRNA (Mouse); ATP-dependent DNA helicase PIF1; DNA repair and recombination helicase PIF1; Pif1/Rrm3 DNA helicase-like protein; PIF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PIF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 650

• Applicable Applications for PIF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PIF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIF1 sirna

siRNA to inhibit PIF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27369615
• NCBI GeneID: 208084
• NCBI Accession #: NP_766041.1
• NCBI GenBank Nucleotide #: NM_172453.3
• UniProt Accession #: Q80SX8; Q80W44; Q8BIZ3; Q8BJ72
• Molecular Weight: 63,731 Da
• NCBI Official Full Name: ATP-dependent DNA helicase PIF1
• NCBI Official Synonym Full Names: PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae)
• NCBI Official Symbol: Pif1
• NCBI Official Synonym Symbols: AI449441; 4631410M14
• NCBI Protein Information: ATP-dependent DNA helicase PIF1
• UniProt Protein Name: ATP-dependent DNA helicase PIF1
• Protein Family: Transcription factor
• UniProt Gene Name: Pif1
• UniProt Entry Name: PIF1_MOUSE

Uniprot Description

PIF1: DNA-dependent ATPase and DNA helicase inhibiting telomerase activity by unwinding DNA/RNA duplex formed by telomerase RNA and telomeric DNA in a 5' to 3' polarity. Negatively regulates telomere length and such inhibition requires its ATPase activity. Tightly cell cycle regulated and expressed in late S/G2 phase. Belongs to the helicase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; DNA-binding; Helicase

Cellular Component: mitochondrion; nuclear chromosome, telomeric region; nucleus

Molecular Function: DNA helicase activity; DNA binding; hydrolase activity; ATP-dependent 5'-3' DNA helicase activity; telomeric DNA binding; nucleotide binding; magnesium ion binding; single-stranded DNA-dependent ATP-dependent DNA helicase activity; helicase activity; ATP-dependent 5'-3' DNA/RNA helicase activity; ATP binding

Biological Process: negative regulation of telomerase activity; regulation of telomere maintenance; DNA repair; telomere maintenance; response to DNA damage stimulus; DNA duplex unwinding; DNA recombination