PDX1 sirna

PDX1 siRNA (Mouse)

Cat. Num. AAA8222067

• Gene Names: Pdx1; Ipf1; IDX-1; IPF-1; Mody4; STF-1; pdx-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDX1; PDX1 siRNA (Mouse); IPF1; Pancreas/duodenum homeobox protein 1; Insulin promoter factor 1; IPF-1; Islet/duodenum homeobox 1; IDX-1; Somatostatin-transactivating factor 1; STF-1; PDX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PDX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 284

• Applicable Applications for PDX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDX1 sirna

siRNA to inhibit PDX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679269
• NCBI GeneID: 18609
• NCBI Accession #: NP_032840.1
• NCBI GenBank Nucleotide #: NM_008814.3
• UniProt Accession #: P52946; Q3ZB03
• Molecular Weight: 30,999 Da
• NCBI Official Full Name: pancreas/duodenum homeobox protein 1
• NCBI Official Synonym Full Names: pancreatic and duodenal homeobox 1
• NCBI Official Symbol: Pdx1
• NCBI Official Synonym Symbols: Ipf1; IDX-1; IPF-1; Mody4; STF-1; pdx-1
• NCBI Protein Information: pancreas/duodenum homeobox protein 1
• UniProt Protein Name: Pancreas/duodenum homeobox protein 1
• Protein Family: Pyridoxal 5'-phosphate synthase
• UniProt Gene Name: Pdx1
• UniProt Synonym Gene Names: Ipf1; IPF-1; IDX-1; STF-1
• UniProt Entry Name: PDX1_MOUSE

Uniprot Description

PDX1: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP. Interacts with the methyltransferase SETD7. Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.

Protein type: DNA-binding; Transcription factor

Cellular Component: cytoplasm; nuclear speck; intracellular; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; multicellular organismal development; positive regulation of transcription, DNA-dependent; exocrine pancreas development; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of cell proliferation; detection of glucose; morphogenesis of embryonic epithelium; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; pancreas development; response to glucose stimulus; cell differentiation; positive regulation of DNA binding; smoothened signaling pathway; transcription, DNA-dependent; positive regulation of insulin secretion; glucose metabolic process; stem cell differentiation; liver development; endocrine pancreas development; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; gut development; insulin secretion; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nitric oxide mediated signal transduction