PA2G4 sirna

PA2G4 siRNA (Mouse)

Cat. Num. AAA8228273

• Gene Names: Pa2g4; Ebp1; 38kDa; Plfap; AA672939
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PA2G4; PA2G4 siRNA (Mouse); EBP1; PLFAP; Proliferation-associated protein 2G4; IRES-specific cellular trans-acting factor 45 kDa; ITAF45; Mpp1; Proliferation-associated protein 1; Protein p38-2G4; PA2G4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PA2G4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 394

• Applicable Applications for PA2G4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PA2G4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PA2G4 sirna

siRNA to inhibit PA2G4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755100
• NCBI GeneID: 18813
• NCBI Accession #: NP_035249.1
• NCBI GenBank Nucleotide #: NM_011119.3
• UniProt Accession #: P50580
• Molecular Weight: 37,971 Da
• NCBI Official Full Name: proliferation-associated protein 2G4
• NCBI Official Synonym Full Names: proliferation-associated 2G4
• NCBI Official Symbol: Pa2g4
• NCBI Official Synonym Symbols: Ebp1; 38kDa; Plfap; AA672939
• NCBI Protein Information: proliferation-associated protein 2G4
• UniProt Protein Name: Proliferation-associated protein 2G4
• Protein Family: Proliferation-associated protein
• UniProt Gene Name: Pa2g4
• UniProt Synonym Gene Names: Ebp1; Plfap; ITAF45
• UniProt Entry Name: PA2G4_MOUSE

Uniprot Description

Ebp1: May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Belongs to the peptidase M24 family.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Nucleolus

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: DNA binding; RNA binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: regulation of translation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; positive regulation of cell differentiation; rRNA processing; negative regulation of apoptosis