NPR2 sirna

NPR2 siRNA (Mouse)

Cat. Num. AAA8236095

• Gene Names: Npr2; cn; pwe; GC-B; GC-B2; GC-B3; mNPR-B
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NPR2; NPR2 siRNA (Mouse); Atrial natriuretic peptide receptor 2; Atrial natriuretic peptide receptor type B; ANP-B; ANPR-B; NPR-B; Guanylate cyclase B; GC-B; NPR2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NPR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 418

• Applicable Applications for NPR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NPR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NPR2 sirna

siRNA to inhibit NPR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28916675
• NCBI GeneID: 230103
• NCBI Accession #: NP_776149.1
• NCBI GenBank Nucleotide #: NM_173788.3
• UniProt Accession #: Q6VVW5; Q6VVW3; Q6VVW4; Q8CGA9; B1AWI8
• Molecular Weight: 46,443 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 2
• NCBI Official Synonym Full Names: natriuretic peptide receptor 2
• NCBI Official Symbol: Npr2
• NCBI Official Synonym Symbols: cn; pwe; GC-B; GC-B2; GC-B3; mNPR-B
• NCBI Protein Information: atrial natriuretic peptide receptor 2
• UniProt Protein Name: Atrial natriuretic peptide receptor 2
• Protein Family: Nitrogen permease regulator
• UniProt Gene Name: Npr2
• UniProt Synonym Gene Names: ANP-B; ANPR-B; NPR-B; GC-B
• UniProt Entry Name: ANPRB_MOUSE

Uniprot Description

ANPB: natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. 21 mutations linked to acromesomelic dysplasia (skeletal malformation). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, RGC; Receptor, misc.; EC 4.6.1.2; Nucleotide Metabolism - purine; Kinase, protein; Guanylyl cyclase; Lyase; Membrane protein, integral; Protein kinase, dual-specificity (receptor); RGC group; RGC family

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: identical protein binding; guanylate cyclase activity; phosphorus-oxygen lyase activity; hormone binding; GTP binding; natriuretic peptide receptor activity; peptide hormone binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; lyase activity; ATP binding; protein kinase activity

Biological Process: cGMP biosynthetic process; ossification; cyclic nucleotide biosynthetic process; receptor guanylyl cyclase signaling pathway; negative regulation of meiotic cell cycle; signal transduction; protein amino acid phosphorylation