NPC1 sirna

NPC1 siRNA (Mouse)

Cat. Num. AAA8226547

• Gene Names: Npc1; spm; lcsd; C85354; nmf164; D18Ertd139e; D18Ertd723e; A430089E03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NPC1; NPC1 siRNA (Mouse); Niemann-Pick C1 protein; NPC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NPC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,277

• Applicable Applications for NPC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NPC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NPC1 sirna

siRNA to inhibit NPC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 89242146
• NCBI GeneID: 18145
• NCBI Accession #: NP_032746.2
• NCBI GenBank Nucleotide #: NM_008720.2
• UniProt Accession #: O35604; O35605; G3X8W9
• Molecular Weight: 142,885 Da
• NCBI Official Full Name: Niemann-Pick C1 protein
• NCBI Official Synonym Full Names: Niemann-Pick type C1
• NCBI Official Symbol: Npc1
• NCBI Official Synonym Symbols: spm; lcsd; C85354; nmf164; D18Ertd139e; D18Ertd723e; A430089E03Rik
• NCBI Protein Information: Niemann-Pick C1 protein
• UniProt Protein Name: Niemann-Pick C1 protein
• Protein Family: Niemann-Pick C1 protein
• UniProt Gene Name: Npc1
• UniProt Entry Name: NPC1_MOUSE

Uniprot Description

NPC1: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. Defects in NPC1 are the cause of Niemann-Pick disease type C1 (NPC1). A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C1 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. An allelic variant of Niemann-Pick disease type C1 is found in people with Nova Scotia ancestry. Patients with the Nova Scotian clinical variant are less severely affected. Belongs to the patched family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; lysosomal membrane; lysosome; integral to plasma membrane; endoplasmic reticulum; extracellular region; integral to membrane; nuclear envelope; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; vesicle; endosome

Molecular Function: protein binding; hedgehog receptor activity; cholesterol binding

Biological Process: steroid metabolic process; response to drug; cholesterol metabolic process; cholesterol transport; protein amino acid glycosylation; cholesterol efflux; endocytosis; adult walking behavior; negative regulation of macroautophagy; cholesterol homeostasis; response to cadmium ion; autophagy; lipid metabolic process; lipid raft organization and biogenesis