NOL9 sirna

NOL9 siRNA (Mouse)

Cat. Num. AAA8220170

• Gene Names: Nol9; AI449622; AW490720; 4632412I24Rik; 6030462G04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOL9; NOL9 siRNA (Mouse); Polynucleotide 5'-hydroxyl-kinase NOL9; Nucleolar protein 9; NOL9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NOL9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 714

• Applicable Applications for NOL9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NOL9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOL9 sirna

siRNA to inhibit NOL9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 227430358
• NCBI GeneID: 74035
• NCBI Accession #: NP_001153071.1
• NCBI GenBank Nucleotide #: NM_001159599.2
• UniProt Accession #: Q3TZX8; Q05A31; Q8BQE1; Q8C8L5; Q8C9A8; Q9D678; A0PJC7; B1AS65
• Molecular Weight: 70,969 Da
• NCBI Official Full Name: polynucleotide 5'-hydroxyl-kinase NOL9 isoform 1
• NCBI Official Synonym Full Names: nucleolar protein 9
• NCBI Official Symbol: Nol9
• NCBI Official Synonym Symbols: AI449622; AW490720; 4632412I24Rik; 6030462G04Rik
• NCBI Protein Information: polynucleotide 5'-hydroxyl-kinase NOL9
• UniProt Protein Name: Polynucleotide 5'-hydroxyl-kinase NOL9
• Protein Family: Polynucleotide 5'-hydroxyl-kinase
• UniProt Gene Name: Nol9
• UniProt Entry Name: NOL9_MOUSE

Uniprot Description

NOL9: Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA. Belongs to the Clp1 family. NOL9/GRC3 subfamily.

Protein type: EC 2.7.1.-; Nucleolus

Cellular Component: membrane; nucleus

Molecular Function: phosphatidylinositol phosphate kinase activity; cobinamide kinase activity; cyclin-dependent protein kinase activating kinase regulator activity; RNA binding; ATP-dependent polynucleotide kinase activity; inositol tetrakisphosphate 2-kinase activity; LPPG:FO 2-phospho-L-lactate transferase activity; phosphorylase kinase regulator activity; cyclin-dependent protein kinase regulator activity; cytidine kinase activity; aminoglycoside phosphotransferase activity; ATP binding; eukaryotic elongation factor-2 kinase activator activity; phenol kinase activity; (S)-lactate 2-kinase activity; glycerate 2-kinase activity; nucleotide binding; GTP-dependent polynucleotide kinase activity; phosphoinositide 3-kinase activity; transferase activity; heptose 7-phosphate kinase activity; phytol kinase activity; eukaryotic elongation factor-2 kinase regulator activity; kinase activity; phosphofructokinase activity; polynucleotide kinase activity

Biological Process: cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of 5.8S rRNA; phosphorylation; rRNA processing