NKX3-1 sirna

NKX3-1 siRNA (Mouse)

Cat. Num. AAA8210830

• Gene Names: Nkx3-1; Bax; NKX3A; NKX3.1; Nkx-3.1; bagpipe
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NKX3-1; NKX3-1 siRNA (Mouse); NKX-3.1; NKX3A; Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NKX3-1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NKX3-1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 237

• Applicable Applications for NKX3-1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NKX3-1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NKX3-1 sirna

siRNA to inhibit NKX3-1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7110673
• NCBI GeneID: 18095
• NCBI Accession #: NP_035051.1
• NCBI GenBank Nucleotide #: NM_010921.3
• UniProt Accession #: P97436; O09087
• Molecular Weight: 26,824 Da
• NCBI Official Full Name: homeobox protein Nkx-3.1
• NCBI Official Synonym Full Names: NK-3 transcription factor, locus 1 (Drosophila)
• NCBI Official Symbol: Nkx3-1
• NCBI Official Synonym Symbols: Bax; NKX3A; NKX3.1; Nkx-3.1; bagpipe
• NCBI Protein Information: homeobox protein Nkx-3.1
• UniProt Protein Name: Homeobox protein Nkx-3.1
• Protein Family: Homeobox protein
• UniProt Gene Name: Nkx3-1
• UniProt Synonym Gene Names: Nkx-3.1; Nkx3a
• UniProt Entry Name: NKX31_MOUSE

Uniprot Description

NKX3-1: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation. By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen- independent phase. Highly expressed in the prostate and, at a lower level, in the testis. Belongs to the NK-3 homeobox family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; DNA-binding; Transcription factor

Cellular Component: intracellular; nucleus

Molecular Function: protein binding; androgen receptor activity; estrogen receptor activity; protein self-association; DNA binding; sequence-specific DNA binding; histone deacetylase binding; estrogen receptor binding; caspase activator activity; protein kinase activator activity; transcription factor binding; transcription factor activity

Biological Process: prostate gland development; transcription from RNA polymerase II promoter; salivary gland development; multicellular organismal development; positive regulation of transcription, DNA-dependent; negative regulation of insulin-like growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of mitotic cell cycle; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; protein kinase B signaling cascade; negative regulation of mitotic cell cycle; negative regulation of epithelial cell proliferation; caspase activation; transcription, DNA-dependent; response to testosterone stimulus; positive regulation of phosphoinositide 3-kinase cascade; internal genitalia morphogenesis; branching morphogenesis of a tube; positive regulation of cell division; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; urogenital system development