Rabbit anti-Mouse Nkx3-1 Polyclonal Antibody | anti-Nkx3-1 antibody

Nkx3-1, ID (Nkx3-1, Nkx-3.1, Nkx3a, Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A) (Azide free) (HRP)

Cat. Num. AAA6325624

• Gene Names: Nkx3-1; Bax; NKX3A; NKX3.1; Nkx-3.1; bagpipe
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Nkx3-1; Polyclonal Antibody; ID (Nkx3-1; Nkx-3.1; Nkx3a; Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A) (Azide free) (HRP); Homeobox protein NK-3 homolog A; anti-Nkx3-1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Nkx3-1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Nkx3-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of mouse Nkx3-1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Nkx3-1 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Nkx3-1 antibody detected the Nkx3-1 protein (arrow).)

Related Product Information for anti-Nkx3-1 antibody

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor (By similarity). Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to suppress growth and tumorigenicity of prostate carcinoma cells. Play a role in the formation of minor salivary glands (particularly palatine and lingual glands). Essential for appropriate differentiation and secretory function of the bulbourethral gland.

Product Categories/Family for anti-Nkx3-1 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Khalili, M., et al. Am. J. Pathol. 176(5):2259-2268(2010). Guo, G., et al. Dev. Cell 18(4):675-685(2010). Thomsen, M.K., et al. Cancer Res. 70(3):979-987(2010). Iwata, T., et al. PLoS ONE 5 (2), E9427 (2010): Sun, Q., et al. J. Biol. Chem. 284(47):32582-32590(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 7110673
• NCBI GeneID: 18095
• NCBI Accession #: NP_035051.1
• NCBI GenBank Nucleotide #: NM_010921.3
• UniProt Accession #: P97436; O09087
• Molecular Weight: 26,824 Da
• NCBI Official Full Name: homeobox protein Nkx-3.1
• NCBI Official Synonym Full Names: NK-3 transcription factor, locus 1 (Drosophila)
• NCBI Official Symbol: Nkx3-1
• NCBI Official Synonym Symbols: Bax; NKX3A; NKX3.1; Nkx-3.1; bagpipe
• NCBI Protein Information: homeobox protein Nkx-3.1; homeobox protein NK-3 homolog A; Drosophila NK-3 transcription factor, locus 1
• UniProt Protein Name: Homeobox protein Nkx-3.1
• Protein Family: Homeobox protein
• UniProt Gene Name: Nkx3-1
• UniProt Synonym Gene Names: Nkx-3.1; Nkx3a
• UniProt Entry Name: NKX31_MOUSE

Uniprot Description

NKX3-1: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation. By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen- independent phase. Highly expressed in the prostate and, at a lower level, in the testis. Belongs to the NK-3 homeobox family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Tumor suppressor; DNA-binding

Cellular Component: intracellular; nucleus

Molecular Function: protein binding; androgen receptor activity; estrogen receptor activity; DNA binding; protein self-association; sequence-specific DNA binding; histone deacetylase binding; estrogen receptor binding; caspase activator activity; protein kinase activator activity; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; prostate gland development; salivary gland development; positive regulation of transcription, DNA-dependent; multicellular organismal development; negative regulation of insulin-like growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of mitotic cell cycle; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; protein kinase B signaling cascade; negative regulation of mitotic cell cycle; negative regulation of epithelial cell proliferation; caspase activation; transcription, DNA-dependent; response to testosterone stimulus; positive regulation of phosphoinositide 3-kinase cascade; internal genitalia morphogenesis; branching morphogenesis of a tube; positive regulation of protein kinase activity; positive regulation of cell division; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; urogenital system development