NKX2-6 sirna

NKX2-6 siRNA (Mouse)

Cat. Num. AAA8200172

• Gene Names: Nkx2-6; Tix; Nkx2.6; tinman; Nkx-2.6; AV044928
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NKX2-6; NKX2-6 siRNA (Mouse); NKX-2.6; NKX2F; Homeobox protein Nkx-2.6; Homeobox protein NK-2 homolog F; NKX2-6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NKX2-6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 289

• Applicable Applications for NKX2-6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NKX2-6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NKX2-6 sirna

siRNA to inhibit NKX2-6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 326807002
• NCBI GeneID: 18092
• NCBI Accession #: NP_035050.2
• NCBI GenBank Nucleotide #: NM_010920.2
• UniProt Accession #: P43688
• Molecular Weight: 31,597 Da
• NCBI Official Full Name: homeobox protein Nkx-2.6
• NCBI Official Synonym Full Names: NK2 homeobox 6
• NCBI Official Symbol: Nkx2-6
• NCBI Official Synonym Symbols: Tix; Nkx2.6; tinman; Nkx-2.6; AV044928
• NCBI Protein Information: homeobox protein Nkx-2.6
• UniProt Protein Name: Homeobox protein Nkx-2.6
• Protein Family: Homeobox protein
• UniProt Gene Name: Nkx2-6
• UniProt Synonym Gene Names: Nkx-2.6; Nkx2f
• UniProt Entry Name: NKX26_MOUSE

Uniprot Description

NKX2-6: In conjunction with NKX2-5, may play a role in both pharyngeal and cardiac embryonic development. Defects in NKX2-6 may be a cause of conotruncal heart malformations (CTHM). CTHM defines a group of congenital heart defects involving the outflow tracts. Examples include truncus arteriosus communis, double-outlet right ventricle and transposition of great arteries. Truncus arteriosus communis is characterized by a single outflow tract instead of a separate aorta and pulmonary artery. In transposition of the great arteries, the aorta arises from the right ventricle and the pulmonary artery from the left ventricle. In double outlet of the right ventricle, both the pulmonary artery and aorta arise from the right ventricle. Belongs to the NK-2 homeobox family.

Protein type: DNA-binding

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: pharyngeal system development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; heart development; multicellular organismal development; positive regulation of cell proliferation; embryonic heart tube development; cell differentiation; negative regulation of apoptosis