NAIP2 sirna

NAIP2 siRNA (Mouse)

Cat. Num. AAA8241176

• Gene Names: Naip2; Birc1b; Naip-rs6
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NAIP2; NAIP2 siRNA (Mouse); BIRC1B; NAIP-RS6; Baculoviral IAP repeat-containing protein 1b; Neuronal apoptosis inhibitory protein 2; NAIP2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NAIP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,447

• Applicable Applications for NAIP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NAIP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NAIP2 sirna

siRNA to inhibit NAIP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 187133243
• NCBI GeneID: 17948
• NCBI Accession #: NP_001119654.1
• NCBI GenBank Nucleotide #: NM_001126182.2
• UniProt Accession #: Q9QUK4; O09124; Q9R030; A9C470
• Molecular Weight: 164,083 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 1b
• NCBI Official Synonym Full Names: NLR family, apoptosis inhibitory protein 2
• NCBI Official Symbol: Naip2
• NCBI Official Synonym Symbols: Birc1b; Naip-rs6
• NCBI Protein Information: baculoviral IAP repeat-containing protein 1b
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 1b
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: Naip2
• UniProt Synonym Gene Names: Birc1b; Naip-rs6
• UniProt Entry Name: BIR1B_MOUSE

Uniprot Description

NAIP2: Sensor component of the NLRC4 inflammasome that specifically recognizes and binds type III secretion system (T3SS) rod proteins such as S.typhimurium (Salmonella) PrgJ and B.thailandensis BsaK from pathogenic bacteria. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri. Prevents motor-neuron apoptosis induced by a variety of signals.

Protein type: Apoptosis

Cellular Component: cytoplasm; nucleus

Molecular Function: metal ion binding; caspase inhibitor activity; nucleotide binding; ubiquitin-protein ligase activity; ATP binding

Biological Process: detection of bacterium; immune system process; apoptosis; defense response to bacterium; cytokinesis; innate immune response; protein ubiquitination; regulation of signal transduction; negative regulation of caspase activity; inflammatory response; negative regulation of apoptosis