MYH9 sirna

MYH9 siRNA (Mouse)

Cat. Num. AAA8227333

• Gene Names: Myh9; Fltn; Myhn1; Myhn-1; TU72.6; NMHCIIA; NMMHC-A; NMMHC-IIA
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYH9; MYH9 siRNA (Mouse); Myosin-9; Cellular myosin heavy chain. type A; Myosin heavy chain 9; Myosin heavy chain. non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa; NMMHC II-a; NMMHC-IIA; MYH9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MYH9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,960

• Applicable Applications for MYH9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MYH9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYH9 sirna

siRNA to inhibit MYH9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 114326446
• NCBI GeneID: 17886
• NCBI Accession #: NP_071855.2
• NCBI GenBank Nucleotide #: NM_022410.3
• UniProt Accession #: Q8VDD5; Q3UHT9; Q3UHU4; Q6KAN6; Q811I2
• Molecular Weight: 226,372 Da
• NCBI Official Full Name: myosin-9
• NCBI Official Synonym Full Names: myosin, heavy polypeptide 9, non-muscle
• NCBI Official Symbol: Myh9
• NCBI Official Synonym Symbols: Fltn; Myhn1; Myhn-1; TU72.6; NMHCIIA; NMMHC-A; NMMHC-IIA
• NCBI Protein Information: myosin-9
• UniProt Protein Name: Myosin-9
• Protein Family: Myosin
• UniProt Gene Name: Myh9
• UniProt Synonym Gene Names: NMMHC-A; NMMHC II-a; NMMHC-IIA
• UniProt Entry Name: MYH9_MOUSE

Uniprot Description

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Actin-binding; Motility/polarity/chemotaxis

Cellular Component: cortical cytoskeleton; signalosome; actomyosin; immunological synapse; cytosol; cell-cell adherens junction; membrane; cytoplasm; uropod; neuromuscular junction; integrin complex; myosin complex; cleavage furrow; protein complex; myosin II complex; leading edge; contractile ring; cell cortex; actin cytoskeleton; ruffle; cytoskeleton; stress fiber; plasma membrane; spindle; nucleus

Molecular Function: microfilament motor activity; actin filament binding; calmodulin binding; protein binding; protein homodimerization activity; protein anchor; ATPase activity; nucleotide binding; motor activity; actin binding; actin-dependent ATPase activity; ADP binding; ATP binding

Biological Process: cellular morphogenesis during differentiation; blood vessel endothelial cell migration; actin filament-based movement; in utero embryonic development; membrane protein ectodomain proteolysis; actomyosin structure organization and biogenesis; cytokinesis; uropod organization and biogenesis; establishment of meiotic spindle localization; regulation of cell shape; cell-cell adhesion; protein transport; actin cytoskeleton reorganization; establishment of T cell polarity; angiogenesis; platelet formation; cell adhesion; cell motility; myoblast fusion; meiotic spindle organization and biogenesis