Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Endothelin 1 (ET-1) Standard Curve.)
Rat Endothelin 1 (ET-1) ELISA Kit | ET-1 elisa kit
Rat Endothelin 1 (ET-1) ELISA Kit
Gene Names
EDN1; ET-1
Reactivity
Rat
Synonyms
Endothelin 1 (ET-1); Rat Endothelin 1 (ET-1) ELISA Kit; EDN1; ET1; HDLCQ7; preproendothelin 1; ET-1 elisa kit
Reactivity
Rat
Specificity
Rat Endothelin 1 (ET-1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat EDN1. No significant cross-reactivity or interference between Rat EDN1 and analogues was observed.
Sequence Length
202
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Endothelin 1 (ET-1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Endothelin 1 (ET-1) Standard Curve.)
Related Product Information for ET-1 elisa kit
Description: This Rat Endothelin 1 (ET-1) ELISA Kit employs a two-site sandwich ELISA to quantitate EDN1 in samples. An antibody specific for EDN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EDN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EDN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EDN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Endothelin 1 (ET-1) gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis.
Background: Endothelin 1 (ET-1) gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
endothelin-1
NCBI Official Symbol
EDN1
NCBI Official Synonym Symbols
ET-1
NCBI Protein Information
endothelin-1
UniProt Protein Name
Endothelin-1
UniProt Gene Name
EDN1
UniProt Synonym Gene Names
ET-1; PPET1
Uniprot Description
Endothelins are endothelium-derived vasoconstrictor peptides.
Research Articles on ET-1
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Product Notes
The Rat ET-1 edn1 (Catalog #AAA9712403) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712403 ELISA Kit recognizes Rat ET-1. It is sometimes possible for the material contained within the vial of "Endothelin 1 (ET-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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