MX1 sirna

MX1 siRNA (Mouse)

Cat. Num. AAA8209723

• Gene Names: Mx1; Mx; Mx-1; AI893580
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MX1; MX1 siRNA (Mouse); Interferon-induced GTP-binding protein Mx1; Influenza resistance protein; Myxoma resistance protein 1; Myxovirus resistance protein 1; MX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 631

• Applicable Applications for MX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MX1 sirna

siRNA to inhibit MX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6996930
• NCBI GeneID: 17857
• NCBI Accession #: NP_034976.1
• NCBI GenBank Nucleotide #: NM_010846.1
• UniProt Accession #: P09922
• Molecular Weight: 72,038 Da
• NCBI Official Full Name: interferon-induced GTP-binding protein Mx1
• NCBI Official Synonym Full Names: MX dynamin-like GTPase 1
• NCBI Official Symbol: Mx1
• NCBI Official Synonym Symbols: Mx; Mx-1; AI893580
• NCBI Protein Information: interferon-induced GTP-binding protein Mx1
• UniProt Protein Name: Interferon-induced GTP-binding protein Mx1
• Protein Family: Interferon-induced GTP-binding protein
• UniProt Gene Name: Mx1
• UniProt Entry Name: MX1_MOUSE

NCBI Description

This gene encodes a member of the Mx protein family of large GTPases, and functions in the innate immunity system. Interferon alpha/beta treatment or viral infection induces expression of this protein, which subsequently accumulates in the cytoplasm and inhibits viral replication. It has been shown to confer resistance to the influenza virus. This gene produces a functional protein in some feral mouse strains, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a deletion or a nonsense mutation that results in a non-functional gene product. [provided by RefSeq, Aug 2015]

Uniprot Description

MX1: Dynamin-like GTPase acting as a cell-autonomous host restriction factor against many viral pathogens. Shows activity against influenza viruses, rhabdovirus VSV, bunyavirus LACV, Thogoto virus, measles virus, Hantaan virus, Coxsackie virus CVB, Rift Valley fever virus RVFV, hepatitis B virus HBV, and Crimean- Congo hemorrhagic fever virus CCHFV. Belongs to the dynamin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Hydrolase

Cellular Component: nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; nucleotide binding

Biological Process: immune system process; response to virus; innate immune response; defense response to virus