MTO1 sirna

MTO1 siRNA (Mouse)

Cat. Num. AAA8240179

• Gene Names: Mto1; 2310039H01Rik; 5730419A02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTO1; MTO1 siRNA (Mouse); Protein MTO1 homolog mitochondrial; MTO1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MTO1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 669

• Applicable Applications for MTO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MTO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MTO1 sirna

siRNA to inhibit MTO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 171460946
• NCBI GeneID: 68291
• NCBI Accession #: NP_080934.2
• NCBI GenBank Nucleotide #: NM_026658.2
• UniProt Accession #: Q923Z3; Q9D2Q5
• Molecular Weight: 74,332 Da
• NCBI Official Full Name: protein MTO1 homolog, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial translation optimization 1 homolog (S. cerevisiae)
• NCBI Official Symbol: Mto1
• NCBI Official Synonym Symbols: 2310039H01Rik; 5730419A02Rik
• NCBI Protein Information: protein MTO1 homolog, mitochondrial
• UniProt Protein Name: Protein MTO1 homolog, mitochondrial
• Protein Family: Mitochondrial translation optimization protein
• UniProt Gene Name: Mto1
• UniProt Entry Name: MTO1_MOUSE

Uniprot Description

MTO1: Involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs. Defects in MTO1 are the cause of combined oxidative phosphorylation deficiency type 10 (COXPD10). An autosomal recessive disorder resulting in variable defects of mitochondrial oxidative respiration. Affected individuals present in infancy with hypertrophic cardiomyopathy and lactic acidosis. The severity is variable, but can be fatal in the most severe cases. Belongs to the MnmG family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing

Cellular Component: mitochondrion

Molecular Function: FAD binding

Biological Process: tRNA wobble uridine modification; tRNA processing