MTMR2 sirna

MTMR2 siRNA (Mouse)

Cat. Num. AAA8224981

• Gene Names: Mtmr2; 6030445P13Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTMR2; MTMR2 siRNA (Mouse); Myotubularin-related protein 2; MTMR2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MTMR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 643

• Applicable Applications for MTMR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MTMR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MTMR2 sirna

siRNA to inhibit MTMR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194394153
• NCBI GeneID: 77116
• NCBI Accession #: NP_076347.3
• NCBI GenBank Nucleotide #: NM_023858.3
• UniProt Accession #: Q9Z2D1; Q8VHA7; B8JJF4
• Molecular Weight: 73,232 Da
• NCBI Official Full Name: myotubularin-related protein 2
• NCBI Official Synonym Full Names: myotubularin related protein 2
• NCBI Official Symbol: Mtmr2
• NCBI Official Synonym Symbols: 6030445P13Rik
• NCBI Protein Information: myotubularin-related protein 2
• UniProt Protein Name: Myotubularin-related protein 2
• Protein Family: Myotubularin-related protein
• UniProt Gene Name: Mtmr2
• UniProt Entry Name: MTMR2_MOUSE

Uniprot Description

MTMR2: Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. Homooligomer and heterooligomer. Interacts with SBF1 and SBF2. interaction with SBF1 increases phosphatase activity. Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.

Protein type: Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.64; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); Cofactor and Vitamin Metabolism - thiamine; Carbohydrate Metabolism - fructose and mannose; Phosphatase, lipid; EC 3.1.3.95

Cellular Component: synaptic vesicle; membrane; axon; dendrite; vacuolar membrane; cytoplasm; dendritic spine; cytosol; nucleus; endosome

Molecular Function: phosphatidylinositol-3-phosphatase activity; protein homodimerization activity; hydrolase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity

Biological Process: myelin formation; dephosphorylation; negative regulation of myelination; inositol phosphate dephosphorylation; negative regulation of endocytosis; neuron development; phosphoinositide dephosphorylation; lipid metabolic process; phosphatidylinositol metabolic process; protein tetramerization; negative regulation of receptor internalization