MTHFD1 sirna

MTHFD1 siRNA (Mouse)

Cat. Num. AAA8225093

• Gene Names: Mthfd1; Dcs; Mthfd; E430024A07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTHFD1; MTHFD1 siRNA (Mouse); C-1-tetrahydrofolate synthase cytoplasmic; C1-THF synthase; MTHFD1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MTHFD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 935

• Applicable Applications for MTHFD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MTHFD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MTHFD1 sirna

siRNA to inhibit MTHFD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 261878543
• NCBI GeneID: 108156
• NCBI Accession #: NP_620084.2
• NCBI GenBank Nucleotide #: NM_138745.2
• UniProt Accession #: Q922D8; Q8R013
• Molecular Weight: 101,200 Da
• NCBI Official Full Name: C-1-tetrahydrofolate synthase, cytoplasmic
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
• NCBI Official Symbol: Mthfd1
• NCBI Official Synonym Symbols: Dcs; Mthfd; E430024A07Rik
• NCBI Protein Information: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Protein Name: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Gene Name: Mthfd1
• UniProt Synonym Gene Names: C1-THF synthase
• UniProt Entry Name: C1TC_MOUSE

NCBI Description

This gene encodes a trifunctional cytoplasmic enzyme. The encoded protein functions as a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase, and a formyltetrahydrofolate synthase. The encoded enzyme functions in de novo synthesis of purines and thymidylate and in regeneration of methionine from homocysteine. [provided by RefSeq, Oct 2009]

Uniprot Description

MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.

Protein type: EC 3.5.4.9; Hydrolase; Cofactor and Vitamin Metabolism - one carbon pool by folate; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.5.1.5; EC 6.3.4.3; Oxidoreductase; Ligase

Cellular Component: mitochondrion; membrane; cytoplasm; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; hydrolase activity; nucleotide binding; oxidoreductase activity; formate-tetrahydrofolate ligase activity; catalytic activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; ligase activity

Biological Process: histidine biosynthetic process; folic acid and derivative biosynthetic process; metabolic process; methionine biosynthetic process; one-carbon compound metabolic process; amino acid biosynthetic process; purine nucleotide biosynthetic process