MINPP1 sirna

MINPP1 siRNA (Mouse)

Cat. Num. AAA8206622

• Gene Names: Minpp1; AA408516
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MINPP1; MINPP1 siRNA (Mouse); MIPP; Multiple inositol polyphosphate phosphatase 1; 2.3-bisphosphoglycerate 3-phosphatase; 2.3-BPG phosphatase; Inositol; 1.3.4.5)-tetrakisphosphate 3-phosphatase; Ins(1.3.4.5)P(4) 3-phosphatase; MINPP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MINPP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 481

• Applicable Applications for MINPP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MINPP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MINPP1 sirna

siRNA to inhibit MINPP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754698
• NCBI GeneID: 17330
• NCBI Accession #: NP_034929.1
• NCBI GenBank Nucleotide #: NM_010799.2
• UniProt Accession #: Q9Z2L6; Q3UA76; Q8VDR0; Q9JJD5
• Molecular Weight: 54,537 Da
• NCBI Official Full Name: multiple inositol polyphosphate phosphatase 1
• NCBI Official Synonym Full Names: multiple inositol polyphosphate histidine phosphatase 1
• NCBI Official Symbol: Minpp1
• NCBI Official Synonym Symbols: AA408516
• NCBI Protein Information: multiple inositol polyphosphate phosphatase 1
• UniProt Protein Name: Multiple inositol polyphosphate phosphatase 1
• Protein Family: Multiple inositol polyphosphate phosphatase
• UniProt Gene Name: Minpp1
• UniProt Synonym Gene Names: Mipp; 2,3-BPG phosphatase; Ins(1,3,4,5)P(4) 3-phosphatase
• UniProt Entry Name: MINP1_MOUSE

Uniprot Description

MINPP1: Acts as a phosphoinositide 5- and phosphoinositide 6- phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3- phosphoglycerate. May play a role in bone development (endochondral ossification). Defects in MINPP1 may be involved in follicular thyroid tumors development. Belongs to the histidine acid phosphatase family. MINPP1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Hydrolase; Secreted; Carbohydrate Metabolism - inositol phosphate; Secreted, signal peptide; EC 3.1.3.62; EC 3.1.3.80

Cellular Component: membrane; endoplasmic reticulum; plasma membrane

Molecular Function: acid phosphatase activity; hydrolase activity; phosphoric monoester hydrolase activity; inositol-1,4,5,6-tetrakisphosphate 6-phosphatase activity; multiple inositol-polyphosphate phosphatase activity; inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity

Biological Process: dephosphorylation