MED1 sirna

MED1 siRNA (Mouse)

Cat. Num. AAA8230575

• Gene Names: Med1; PBP; Pparbp; CRSP210; DRIP205; TRAP220; AI480703; l11Jus15
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MED1; MED1 siRNA (Mouse); CRSP210; DRIP205; PBP; PPARBP; TRAP220; TRIP2; Mediator of RNA polymerase II transcription subunit 1; Mediator complex subunit 1; Peroxisome proliferator-activated receptor-binding protein; PPAR-binding protein; Thyroid hormone receptor-associated protein complex 220 kDa component; Trap220; Thyroid receptor-interacting protein 2; TR-interacting protein 2; TRIP-2; MED1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MED1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1575

• Applicable Applications for MED1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MED1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MED1 sirna

siRNA to inhibit MED1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 121582398
• NCBI GeneID: 19014
• NCBI Accession #: NP_001073587.1
• NCBI GenBank Nucleotide #: NM_001080118.1
• UniProt Accession #: Q925J9; O88323; Q3UHV0; Q6AXD5; Q8BW37; Q8BX19; Q8VDQ7; Q925K0; A2A526; A2A528
• Molecular Weight: 165,450 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 1 isoform 3
• NCBI Official Synonym Full Names: mediator complex subunit 1
• NCBI Official Symbol: Med1
• NCBI Official Synonym Symbols: PBP; Pparbp; CRSP210; DRIP205; TRAP220; AI480703; l11Jus15
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 1
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 1
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: Med1
• UniProt Synonym Gene Names: Crsp210; Drip205; Pbp; Pparbp; Trap220; Trip2; PBP; PPAR-binding protein; Trap220; TR-interacting protein 2; TRIP-2
• UniProt Entry Name: MED1_MOUSE

Uniprot Description

MED1: a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. Also is a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. May regulates p53-dependent apoptosis. Is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; membrane; nucleolus; Srb-mediator complex; nucleus; chromatin; ubiquitin ligase complex

Molecular Function: ligand-dependent nuclear receptor binding; peroxisome proliferator activated receptor binding; retinoic acid receptor binding; chromatin DNA binding; transcription coactivator activity; receptor activity; transcription factor binding; thyroid hormone receptor coactivator activity; protein binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; protein complex binding; LBD domain binding; transcription cofactor activity; estrogen receptor binding; thyroid hormone receptor binding; chromatin binding; nuclear hormone receptor binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; lactation; lens development in camera-type eye; embryonic placenta development; erythrocyte development; positive regulation of transcription, DNA-dependent; heart development; multicellular organismal development; positive regulation of estrogen receptor signaling pathway; cell morphogenesis; positive regulation of mammary gland epithelial cell proliferation; enucleate erythrocyte development; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; androgen biosynthetic process; embryonic hindlimb morphogenesis; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; angiogenesis; positive regulation of keratinocyte differentiation; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transcription from RNA polymerase I promoter; organ regeneration; camera-type eye development; monocyte differentiation; positive regulation of protein import into nucleus, translocation; transcription, DNA-dependent; in utero embryonic development; embryonic hemopoiesis; mammary gland epithelial cell proliferation; thyroid hormone generation; liver development; mRNA transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; keratinocyte differentiation; negative regulation of neuron differentiation; organ morphogenesis; androgen receptor signaling pathway; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of apoptosis