MCPH1 sirna

MCPH1 siRNA (Mouse)

Cat. Num. AAA8233699

• Gene Names: Mcph1; MCT; BRIT1; 5430437K10Rik; D030046N04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MCPH1; MCPH1 siRNA (Mouse); Microcephalin; MCPH1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MCPH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 822

• Applicable Applications for MCPH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MCPH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MCPH1 sirna

siRNA to inhibit MCPH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 37059803
• NCBI GeneID: 244329
• NCBI Accession #: NP_775281.2
• NCBI GenBank Nucleotide #: NM_173189.2
• UniProt Accession #: Q7TT79; Q8BNI9; Q8C9I7
• Molecular Weight: 90,373 Da
• NCBI Official Full Name: microcephalin
• NCBI Official Synonym Full Names: microcephaly, primary autosomal recessive 1
• NCBI Official Symbol: Mcph1
• NCBI Official Synonym Symbols: MCT; BRIT1; 5430437K10Rik; D030046N04Rik
• NCBI Protein Information: microcephalin
• UniProt Protein Name: Microcephalin
• Protein Family: Microcephalin
• UniProt Gene Name: Mcph1
• UniProt Entry Name: MCPH1_MOUSE

Uniprot Description

MCPH1: a DNA damage responsive protein and transcriptional repressor of human telomere reverse transcriptase. An early responder to DNA damage. Required for double strand break repair via homologous recombination and nonhomologous end joining. Promotes chromatin relaxation by recruiting and maintaining the chromatin remodeling SWI-SNF complex at sites of DNA double strand breakage. Participates in the ATR/Chk1-mediated DNA checkpoint in humans and transcriptionally regulates BRCA1 and Chk1. Plays a role in neurogenesis and regulation of the size of the cerebral cortex. Mutations of MCPH1 can cause microcephaly in humans and appears to play an evolutionarily conserved role in brain development. Alterations have been identified in breast, ovarian and prostate cancer. Appears to coordinate S-M transitions in fly embryos.

Protein type: Cell cycle regulation

Cellular Component: cytoskeleton; cell; cytoplasm

Molecular Function: identical protein binding

Biological Process: regulation of centrosome cycle; regulation of kinase activity; establishment of mitotic spindle orientation; regulation of gene expression; regulation of inflammatory response; cerebral cortex development