MCL1 sirna

MCL1 siRNA (Mouse)

Cat. Num. AAA8234207

• Gene Names: Mcl1; Mcl-1; AW556805
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MCL1; MCL1 siRNA (Mouse); Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Bcl-2-related protein EAT/mcl1; MCL1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MCL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 331

• Applicable Applications for MCL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MCL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MCL1 sirna

siRNA to inhibit MCL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678824
• NCBI GeneID: 17210
• NCBI Accession #: NP_032588.1
• NCBI GenBank Nucleotide #: NM_008562.3
• UniProt Accession #: P97287; Q3TUS0; Q792P0; Q9CRI4; D2K6L9
• Molecular Weight: 30,930 Da
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 homolog
• NCBI Official Synonym Full Names: myeloid cell leukemia sequence 1
• NCBI Official Symbol: Mcl1
• NCBI Official Synonym Symbols: Mcl-1; AW556805
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1 homolog
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1 homolog
• Protein Family: Minichromosome loss protein
• UniProt Gene Name: Mcl1
• UniProt Entry Name: MCL1_MOUSE

Uniprot Description

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Channel, misc.; Apoptosis; Membrane protein, integral; Mitochondrial; Inhibitor

Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity; BH3 domain binding

Biological Process: regulation of apoptosis; apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; multicellular organismal development; response to cytokine stimulus; cell differentiation; negative regulation of apoptosis