MC2R sirna

MC2R siRNA (Mouse)

Cat. Num. AAA8228333

• Gene Names: Mc2r; ACTHR; MC2-R; ACTH-R
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MC2R; MC2R siRNA (Mouse); ACTHR; Adrenocorticotropic hormone receptor; ACTH receptor; ACTH-R; Adrenocorticotropin receptor; Melanocortin receptor 2; MC2-R; MC2R sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MC2R siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 296

• Applicable Applications for MC2R sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MC2R gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MC2R sirna

siRNA to inhibit MC2R expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 411147427
• NCBI GeneID: 17200
• NCBI Accession #: NP_001258645.1
• NCBI GenBank Nucleotide #: NM_001271716.1
• UniProt Accession #: Q64326
• Molecular Weight: 33,982 Da
• NCBI Official Full Name: adrenocorticotropic hormone receptor
• NCBI Official Synonym Full Names: melanocortin 2 receptor
• NCBI Official Symbol: Mc2r
• NCBI Official Synonym Symbols: ACTHR; MC2-R; ACTH-R
• NCBI Protein Information: adrenocorticotropic hormone receptor
• UniProt Protein Name: Adrenocorticotropic hormone receptor
• Protein Family: Adrenocorticotropic hormone receptor
• UniProt Gene Name: Mc2r
• UniProt Synonym Gene Names: Acthr; ACTH receptor; ACTH-R; MC2-R
• UniProt Entry Name: ACTHR_MOUSE

Uniprot Description

MC2R: Receptor for ACTH. This receptor is mediated by G proteins (G(s)) which activate adenylate cyclase. Defects in MC2R are the cause of glucocorticoid deficiency type 1 (GCCD1); also known as familial glucocorticoid deficiency type 1 (FGD1). GCCD1 is an autosomal recessive disorder due to congenital insensitivity or resistance to adrenocorticotropin (ACTH). It is characterized by progressive primary adrenal insufficiency, without mineralocorticoid deficiency. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: membrane; cytoplasm; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; melanocortin receptor activity; adrenocorticotropin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of cAMP biosynthetic process; signal transduction