MASTL sirna

MASTL siRNA (Mouse)

Cat. Num. AAA8226634

• Gene Names: Mastl; GW; GWL; THC2; C88295; MAST-L; 2700091H24Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MASTL; MASTL siRNA (Mouse); GW; GWL; Serine/threonine-protein kinase greatwall; Microtubule-associated serine/threonine-protein kinase-like; MAST-L; MASTL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MASTL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 342

• Applicable Applications for MASTL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MASTL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MASTL sirna

siRNA to inhibit MASTL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 254540066
• NCBI GeneID: 67121
• NCBI Accession #: NP_080255.3
• NCBI GenBank Nucleotide #: NM_025979.4
• UniProt Accession #: Q8C0P0; Q3MIA9; Q5RJW0; Q6NXX9; Q8BVF3; Q9CZH9; Q9D9V0; A2AQY2
• Molecular Weight: 38,030 Da
• NCBI Official Full Name: serine/threonine-protein kinase greatwall
• NCBI Official Synonym Full Names: microtubule associated serine/threonine kinase-like
• NCBI Official Symbol: Mastl
• NCBI Official Synonym Symbols: GW; GWL; THC2; C88295; MAST-L; 2700091H24Rik
• NCBI Protein Information: serine/threonine-protein kinase greatwall
• UniProt Protein Name: Serine/threonine-protein kinase greatwall
• Protein Family: Serine/threonine-protein kinase greatwall
• UniProt Gene Name: Mastl
• UniProt Synonym Gene Names: Gw; Gwl; GW; GWL; MAST-L
• UniProt Entry Name: GWL_MOUSE

Uniprot Description

MASTL: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; MAST family

Cellular Component: nucleoplasm; centrosome; cytoskeleton; cytoplasm; nucleus; cleavage furrow

Molecular Function: transferase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: mitosis; anaphase-promoting complex activation during meiotic cell cycle; female meiosis II; cell division; regulation of cell cycle; cell cycle; negative regulation of protein phosphatase type 2A activity; G2/M transition of mitotic cell cycle; phosphorylation; protein amino acid phosphorylation