MAFB sirna

MAFB siRNA (Mouse)

Cat. Num. AAA8202129

• Gene Names: Mafb; kr; Krml; Krml1; Kreisler
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAFB; MAFB siRNA (Mouse); KRML; MAF1; Transcription factor MafB; Maf-B; Kreisler; Segmentation protein Kr; Transcription factor Maf-1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog B; MAFB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAFB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for MAFB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAFB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAFB sirna

siRNA to inhibit MAFB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23308601
• NCBI GeneID: 16658
• NCBI Accession #: NP_034788.1
• NCBI GenBank Nucleotide #: NM_010658.3
• UniProt Accession #: P54841; Q3U3C8; Q3UPT9
• Molecular Weight: 35,809 Da
• NCBI Official Full Name: transcription factor MafB
• NCBI Official Synonym Full Names: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian)
• NCBI Official Symbol: Mafb
• NCBI Official Synonym Symbols: kr; Krml; Krml1; Kreisler
• NCBI Protein Information: transcription factor MafB
• UniProt Protein Name: Transcription factor MafB
• Protein Family: Transcription factor
• UniProt Gene Name: Mafb
• UniProt Synonym Gene Names: Krml; Maf1; Maf-B
• UniProt Entry Name: MAFB_MOUSE

Uniprot Description

MAFB: Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO). MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients. Belongs to the bZIP family. Maf subfamily.

Protein type: Oncoprotein; Tumor suppressor; DNA-binding; Transcription factor

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; rhombomere 6 development; inner ear morphogenesis; negative regulation of erythrocyte differentiation; thymus development; transcription, DNA-dependent; T cell differentiation in the thymus; respiratory gaseous exchange; regulation of transcription from RNA polymerase II promoter; segment specification; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; rhombomere 5 development; brain segmentation